Iron is an essential element for both normal and cancer cells in humans. Iron overload is known to induce some kinds of cancer, which suggests that prevention of iron overload may become a therapeutic strategy for cancer prevention. However, iron depletion mono-therapy by iron decreased treatment has not been thought to be superior to ordinary chemotherapy and is not part of the standard therapeutic strategy for the treatment of cancer. We revealed that iron depletion inhibited the cancer cell proliferation and reciprocally induced angiogenesis in vitro and in vivo. Treatment to deplete iron levels combined with anti-angiogenic therapy could induce a novel therapeutic effect in the treatment of cancer.