論文

査読有り 国際誌
2019年11月18日

Protein phosphatase 2A regulates the nuclear accumulation of the Arabidopsis bZIP protein VIP1 under hypo-osmotic stress.

Journal of experimental botany
  • Daisuke Tsugama
  • ,
  • Hyuk Sung Yoon
  • ,
  • Kaien Fujino
  • ,
  • Shenkui Liu
  • ,
  • Tetsuo Takano

70
21
開始ページ
6101
終了ページ
6112
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/jxb/erz384

VIP1 is a bZIP transcription factor in Arabidopsis thaliana. When cells are exposed to mechanical stress, VIP1 transiently accumulates in the nucleus, where it regulates the expression of its target genes and suppresses mechanical stress-induced root waving. The nuclear-cytoplasmic shuttling of VIP1 is regulated by phosphorylation and calcium-dependent signaling, but specific regulators of these processes remain to be identified. Here, inhibitors of protein phosphatase 2A (PP2A) are shown to inhibit both the mechanical stress-induced dephosphorylation and nuclear accumulation of VIP1. The PP2A B subunit, which recruits substrates of PP2A holoenzyme, is classified into B, B', B'', and B''' families. Using bimolecular fluorescence complementation, in vitro pull-down, and yeast two-hybrid assays, we show that VIP1 interacts with at least two of the six members of the Arabidopsis PP2A B''-family subunit, which have calcium-binding EF-hand motifs. VIP1AAA, a constitutively nuclear-localized VIP1 variant with substitutions in putative phosphorylation sites of VIP1, suppressed the root waving induced by VIP1-SRDX (a repression domain-fused variant of VIP1). These results support the idea that VIP1 is dephosphorylated by PP2A and that the dephosphorylation suppresses the root waving. The phosphorylation sites of VIP1 and its homologs were narrowed down by in vitro phosphorylation, yeast two-hybrid, and protein subcellular localization assays.

リンク情報
DOI
https://doi.org/10.1093/jxb/erz384
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31504762
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859724
ID情報
  • DOI : 10.1093/jxb/erz384
  • PubMed ID : 31504762
  • PubMed Central 記事ID : PMC6859724

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