論文

査読有り
2009年3月

The 14-3-3 Protein GF14c Acts as a Negative Regulator of Flowering in Rice by Interacting with the Florigen Hd3a

PLANT AND CELL PHYSIOLOGY
  • Yekti Asih Purwestri
  • ,
  • Yuka Ogaki
  • ,
  • Shojiro Tamaki
  • ,
  • Hiroyuki Tsuji
  • ,
  • Ko Shimamoto

50
3
開始ページ
429
終了ページ
438
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcp012
出版者・発行元
OXFORD UNIV PRESS

Hd3a and FT proteins have recently been proposed to act as florigens in rice and Arabidopsis, respectively; however, the molecular mechanisms of their function remain to be determined. In this study, we identified GF14c (a 14-3-3 protein) as an Hd3a-interacting protein in a yeast two-hybrid screen. In vitro and in vivo experiments, using a combination of pull-down assays and bimolecular fluorescence complementation, confirmed the interaction between Hd3a and GF14c. Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering. GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions. These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a. Since the 14-3-3 protein has been shown to interact with FT protein in tomato and Arabidopsis, our results in rice provide important findings about FT signaling in plants.

リンク情報
DOI
https://doi.org/10.1093/pcp/pcp012
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19179350
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000264395800001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/pcp/pcp012
  • ISSN : 0032-0781
  • eISSN : 1471-9053
  • PubMed ID : 19179350
  • Web of Science ID : WOS:000264395800001

エクスポート
BibTeX RIS