論文

査読有り
2015年

Pierisins and CARP-1: ADP-Ribosylation of DNA by ART Cs in Butterflies and Shellfish

ENDOGENOUS ADP-RIBOSYLATION
  • Tsuyoshi Nakano
  • ,
  • Azusa Takahashi-Nakaguchi
  • ,
  • Masafumi Yamamoto
  • ,
  • Masahiko Watanabe

384
開始ページ
127
終了ページ
149
記述言語
英語
掲載種別
論文集(書籍)内論文
DOI
10.1007/82_2014_416
出版者・発行元
SPRINGER-VERLAG BERLIN

The cabbage butterfly, Pieris rapae, and related species possess a previously unknown ADP-ribosylating toxin, guanine specific ADP-ribosyltransferase. This enzyme toxin, known as pierisin, consists of enzymatic N-terminal domain and receptor-binding C-terminal domain, or typical AB-toxin structure. Pierisin efficiently transfers an ADP-ribosyl moiety to the N2 position of the guanine base of dsDNA. Receptors for pierisin are suggested to be the neutral glycosphingolipids, globotriaosylceramide (Gb3), and globotetraosylceramide (Gb4). This DNA-modifying toxin exhibits strong cytotoxicity and induces apoptosis in various human cell lines, which can be blocked by Bc1-2. Pierisin also produces detrimental effects on the eggs and larvae of the non-habitual parasitoids. In contrast, a natural parasitoid of the cabbage butterfly, Cotesia glortterata, was resistant to this toxin. The physiological role of pierisin in the butterfly is suggested to be a defense factor against parasitization by wasps. Other type of DNA ADPribosyltransferase is present in certain kinds of edible clams. For example, the CARP-1 protein found in Mere trix latnarckii consists of an enzymatic domain without a possible receptor-binding domain. Pierisin and CARP-1 are almost fully non-homologous at the amino acid sequence level, but other ADP-ribosyltransferases homologous to pierisin are present in different biological species such as eubacterium Streptotnyces. Possible diverse physiological roles of the DNA ADPribosyltransferases are discussed.

Web of Science ® 被引用回数 : 16

リンク情報
DOI
https://doi.org/10.1007/82_2014_416
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25033755
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000361510900008&DestApp=WOS_CPL

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