論文

査読有り
2016年10月

Effect of removing cumulus cells from porcine cumulus-oocyte complexes derived from small and medium follicles during IVM on the apoptotic status and meiotic progression of the oocytes

THERIOGENOLOGY
  • Pilar Ferre
  • ,
  • Tra Mi Thi Bui
  • ,
  • Takuya Wakai
  • ,
  • Hiroaki Funahashi

86
7
開始ページ
1705
終了ページ
1710
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.theriogenology.2016.05.024
出版者・発行元
ELSEVIER SCIENCE INC

The present study was undertaken to examine the apoptotic status and meiotic progression of oocytes from small follicle (SF; 0.5-2 mm in diameter) and medium follicle (MF; 3-6 mm in diameter) when the oocytes were denuded before, during, and after IVM. Cumulus-oocyte complexes (COCs) were collected from SF or MF of prepubertal gilt ovaries. Before or 20 hours after the start of IVM culture, some oocytes were denuded and cultured for IVM. At the end of IVM, apoptotic status and meiotic progression of the oocytes were compared with oocytes matured in the presence of cumulus cells (CCs) by Annexin-V/PI assay and 4',6-Diamidino-2-phenylindole staining. Apoptotic status of the oocytes was only affected by time when the oocytes were denuded. In both oocytes from SF and MF, although the incidence of early and late apoptotic oocytes was significantly higher when the CCs were removed before IVM, the rate was significantly lower when CCs were removed 20 and 44 hours after the start of IVM. The incidence of mature oocytes was significantly affected by both the origin of COCs and time when oocytes were denuded from the COCs. Although the percentage of mature oocytes was higher in MF than SF, maturation rates were significantly higher when oocytes were denuded 20 hours, as compared with 0 and 44 hours after the start of IVM. However, the percentage of mature oocytes with a morphologically normal spindle was significantly higher when oocytes were denuded 44 hours, rather than 22 hours of IVM. In conclusion, removing CCs 20 hours after the start of IVM seems to promote meiotic progression of the oocytes to the metaphase-II stage even when the COCs were collected from SF, although factor(s) from or communication with CCs during IVM may need to obtain a morphologically normal spindle in mature oocytes. (C) 2016 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.theriogenology.2016.05.024
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27329157
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000383935500009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.theriogenology.2016.05.024
  • ISSN : 0093-691X
  • eISSN : 1879-3231
  • PubMed ID : 27329157
  • Web of Science ID : WOS:000383935500009

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