2020年
Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal
Methods in Molecular Biology
- ,
- 巻
- 2050
- 号
- 開始ページ
- 155
- 終了ページ
- 161
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/978-1-4939-9740-4_16
- 出版者・発行元
- Methods in Molecular Biology
The green alga Chlamydomonas reinhardtii has been widely used to study many biological processes, including photosynthesis, flagellar motility, sexual reproduction, metabolism, and genetics. Here, we describe a step-by-step protocol of rapid and efficient transformation method for wild type cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to the transformation of other industrially useful algae including diatom by optimizing the electric conditions.
- リンク情報
- ID情報
-
- DOI : 10.1007/978-1-4939-9740-4_16
- ISSN : 1064-3745
- ISSN : 1940-6029
- ISBN : 9781493997398
- ISBN : 9781493997404
- ORCIDのPut Code : 78804718
- PubMed ID : 31468489
- SCOPUS ID : 85071743582