2014年9月
Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii
Photosynthesis Research
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- 巻
- 121
- 号
- 2-3
- 開始ページ
- 175
- 終了ページ
- 184
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11120-014-9983-x
- 出版者・発行元
- Springer Netherlands
Aquatic microalgae induce a carbon-concentrating mechanism (CCM) to maintain photosynthetic activity in low-CO2 (LC) conditions. Although the molecular mechanism of the CCM has been investigated using the single-cell green alga Chlamydomonas reinhardtii, and several CCM-related genes have been identified by analyzing high-CO2 (HC)-requiring mutants, many aspects of the CO2-signal transduction pathways remain to be elucidated. In this study, we report the isolation of novel HC-requiring mutants defective in the induction of CCM by DNA tagging. Growth rates of 20,000 transformants grown under HC and LC conditions were compared, and three HC-requiring mutants (H24, H82, and P103) were isolated. The photosynthetic CO2-exchange activities of these mutants were significantly decreased compared with that of wild-type cells, and accumulation of HLA3 and both LCIA and HLA3 were absent in mutants H24 and H82, respectively. Although the insertion of the marker gene and the HC-requiring phenotype were linked in the tetrad progeny of H82, and a calcium-sensing receptor CAS was disrupted by the insertion, exogenous expression of CAS alone could not complement the HC-requiring phenotype.
- リンク情報
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- DOI
- https://doi.org/10.1007/s11120-014-9983-x
- CiNii Articles
- http://ci.nii.ac.jp/naid/120005385881
- CiNii Books
- http://ci.nii.ac.jp/ncid/AA00362200
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24549931
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000338763500007&DestApp=WOS_CPL
- URL
- http://link.springer.com/content/pdf/10.1007/s11120-014-9983-x.pdf
- URL
- http://link.springer.com/article/10.1007/s11120-014-9983-x/fulltext.html
- URL
- http://link.springer.com/content/pdf/10.1007/s11120-014-9983-x
- ID情報
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- DOI : 10.1007/s11120-014-9983-x
- ISSN : 0166-8595
- eISSN : 1573-5079
- CiNii Articles ID : 120005385881
- CiNii Books ID : AA00362200
- PubMed ID : 24549931
- Web of Science ID : WOS:000338763500007