2021年8月
Rapid construction of fluorescence quenching-based immunosensor Q-bodies using alpha-helical coiled-coil peptides
CHEMICAL COMMUNICATIONS
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- 巻
- 57
- 号
- 66
- 開始ページ
- 8206
- 終了ページ
- 8209
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/d1cc02605d
- 出版者・発行元
- ROYAL SOC CHEMISTRY
Here, we report a rapid and efficient method to fabricate Quenchbodies (Q-bodies) that can detect targets with antigen-dependent fluorescence augmentation using a stable coiled-coil peptide pair, E4 and K4 (coiled Q-body, CQ-body). The CQ-body allowed antigen detection not only in buffer but also in 50% plasma. Furthermore, we describe FRET-type CQ-bodies using a dual-coloured K4 peptide, which allowed a more precise antigen quantification. Lastly, successful fabrication of nanobody-based CQ-body shows its applicability to a range of antibody fragments.
- リンク情報
- ID情報
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- DOI : 10.1039/d1cc02605d
- ISSN : 1359-7345
- eISSN : 1364-548X
- Web of Science ID : WOS:000678763100001