論文

査読有り
2011年12月

Erythroid/Myeloid Progenitors and Hematopoietic Stem Cells Originate from Distinct Populations of Endothelial Cells

CELL STEM CELL
  • Michael J. Chen
  • ,
  • Yan Li
  • ,
  • Maria Elena De Obaldia
  • ,
  • Qi Yang
  • ,
  • Amanda D. Yzaguirre
  • ,
  • Tomoko Yamada-Inagawa
  • ,
  • Chris S. Vink
  • ,
  • Avinash Bhandoola
  • ,
  • Elaine Dzierzak
  • ,
  • Nancy A. Speck

9
6
開始ページ
541
終了ページ
552
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.stem.2011.10.003
出版者・発行元
CELL PRESS

Hematopoietic stem cells (HSCs) and an earlier wave of definitive erythroid/myeloid progenitors (EMPs) differentiate from hemogenic endothelial cells in the conceptus. EMPs can be generated in vitro from embryonic or induced pluripotent stem cells, but efforts to produce HSCs have largely failed. The formation of both EMPs and HSCs requires the transcription factor Runx1 and its non-DNA binding partner core binding factor beta (CBF beta). Here we show that the requirements for CBF beta in EMP and HSC formation in the conceptus are temporally and spatially distinct. Panendothelial expression of CBF beta in Tek-expressing cells was sufficient for EMP formation, but was not adequate for HSC formation. Expression of CBF beta in Ly6a-expressing cells, on the other hand, was sufficient for HSC, but not EMP, formation. The data indicate that EMPs and HSCs differentiate from distinct populations of hemogenic endothelial cells, with Ly6a expression specifically marking the HSC-generating hemogenic endothelium.

リンク情報
DOI
https://doi.org/10.1016/j.stem.2011.10.003
ID情報
  • DOI : 10.1016/j.stem.2011.10.003

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