2016年12月
Development of a Novel and Rapid Fully Automated Genetic Testing System
ANALYTICAL SCIENCES
ダウンロード
回数 : 90
- 巻
- 32
- 号
- 12
- 開始ページ
- 1375
- 終了ページ
- 1379
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2116/analsci.32.1375
- 出版者・発行元
- JAPAN SOC ANALYTICAL CHEMISTRY
We have developed a rapid genetic testing system integrating nucleic acid extraction, purification, amplification, and detection in a single cartridge. The system performs real-time polymerase chain reaction (PCR) after nucleic acid purification in a fully automated manner. RNase P, a housekeeping gene, was purified from human nasal epithelial cells using silica-coated magnetic beads and subjected to real-time PCR using a novel droplet-real-time-PCR machine. The process was completed within 13 min. This system will be widely applicable for research and diagnostic uses.
- リンク情報
-
- DOI
- https://doi.org/10.2116/analsci.32.1375
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702263207009925
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000390975100019&DestApp=WOS_CPL
- URL
- https://www.jstage.jst.go.jp/article/analsci/32/12/32_1375/_pdf
- ID情報
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- DOI : 10.2116/analsci.32.1375
- ISSN : 0910-6340
- eISSN : 1348-2246
- J-Global ID : 201702263207009925
- Web of Science ID : WOS:000390975100019