論文

査読有り
2020年12月

Resveratrol inhibits Ca2+ signals and aggregation of platelets

Environmental Health and Preventive Medicine
  • Mikio Marumo
  • ,
  • Kazumi Ekawa
  • ,
  • Ichiro Wakabayashi

担当区分
最終著者, 責任著者
25
1
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s12199-020-00905-1
出版者・発行元
Springer Science and Business Media LLC

<title>Abstract</title>
<sec>
<title>Background</title>
Resveratrol has been shown to inhibit platelet aggregation. However, the mechanism for this action of resveratrol remains to be clarified. The purpose of this study was to elucidate the Ca2+-related mechanism for the inhibitory action of resveratrol on platelet aggregation.


</sec>
<sec>
<title>Methods</title>
Ca2+ entry and subsequent aggregation of human platelets induced by different stimulants including thrombin, thapsigargin, and 1-oleoyl-2-acetylglycerol (OAG) were measured by the fluorescence method and light transmittance method, respectively. Each stimulant was added to a nominally Ca2+-free medium containing platelets, and then CaCl2 was added to the medium to induce Ca2+ influx into platelets.


</sec>
<sec>
<title>Results</title>
Thapsigargin-induced Ca2+ entry into platelets and subsequent platelet aggregation were significantly inhibited in the presence of resveratrol at 6.25 μM or higher concentrations, while OAG-induced Ca2+ entry and subsequent platelet aggregation were not affected by resveratrol at concentrations up to 50 μM. In the nominally Ca2+-free medium, thrombin induced a small transient increase in intracellular Ca2+ concentrations, which was attenuated in the presence of resveratrol at 12.5 μM or higher concentrations. Thrombin-induced Ca2+ entry into platelets and subsequent platelet aggregation were significantly inhibited in the presence of resveratrol at 12.5 μM or higher concentrations.


</sec>
<sec>
<title>Conclusions</title>
The results suggest that resveratrol inhibits thrombin-induced platelet aggregation through decreasing Ca2+ release from its stores and inhibiting store-operated Ca2+ influx into platelets.


</sec>

リンク情報
DOI
https://doi.org/10.1186/s12199-020-00905-1
URL
http://link.springer.com/content/pdf/10.1186/s12199-020-00905-1.pdf
URL
http://link.springer.com/article/10.1186/s12199-020-00905-1/fulltext.html

エクスポート
BibTeX RIS