Peptides in the blood of patients suffering from sepsis were inclusively analyzed using the BLOTCHIP―MS method. Twenty patients with sepsis were divided into two groups of patients with and those without a complication of disseminated intravascular coagulation (DIC). By differential analysis of peptides in blood for these groups, we selected thirteen MS peaks as candidate marker peptides for prediction of DIC. By further ROC analysis, eleven sets, each consisting of four peak peptides, were found to show AUC of 1. Among the thirteen peptides, four frequently detected peptides were subjected to precision mass measurement and subsequent MS/MS analysis. Consequently, two peptides of m/z = 2743 and m/z = 2886 were identified to be α2-HS-glycoprotein and fibrinogen β chain, respectively, both of which consist of 27 amino acids. These peptides are expected to be biomarkers for prediction of DIC in patients with sepsis.