論文

査読有り 国際誌
2020年6月14日

CENP-50 is required for papilloma development in the two-stage skin carcinogenesis model.

Cancer science
  • Megumi Saito
  • ,
  • Naoko Kagawa
  • ,
  • Kazuhiro Okumura
  • ,
  • Haruka Munakata
  • ,
  • Eriko Isogai
  • ,
  • Tatsuo Fukagawa
  • ,
  • Yuichi Wakabayashi

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/cas.14533

CENP-50/U is a component of the CENP-O complex (CENP-O/P/Q/R/U) and localizes to the centromere throughout the cell cycle. Aberrant expression of CENP-50/U has been reported in many types of cancers. However, as Cenp-50/U-deficient mice die during early embryogenesis, its functions remain poorly understood in vivo. To investigate the role of Cenp-50/U in skin carcinogenesis, we generated Cenp-50/U conditional knockout (K14CreER -Cenp-50/Ufl/fl ) mice and subjected them to the 7,12-dimethylbenz(a)anthracene (DMBA)/TPA chemical carcinogenesis protocol. As a result, early-stage papillomas decreased in Cenp-50/U-deficient mice. In contrast, Cenp-50/U-deficient mice demonstrated almost the same carcinoma incidence as control mice. Furthermore, mRNA expression analysis using DMBA/TPA-induced papillomas and carcinomas revealed that Cenp-50/U expression levels in papillomas were significantly higher than in carcinomas. These results suggest that Cenp-50/U functions mainly in early papilloma development and it has little effect on malignant conversion.

リンク情報
DOI
https://doi.org/10.1111/cas.14533
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32535988

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