2009年8月
Proteomic analysis of the G-layer in poplar tension wood
JOURNAL OF WOOD SCIENCE
- ,
- ,
- ,
- ,
- 巻
- 55
- 号
- 4
- 開始ページ
- 250
- 終了ページ
- 257
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s10086-009-1032-6
- 出版者・発行元
- SPRINGER TOKYO
Angiosperm trees bend their stems by forming tension wood at the upper side of leaning stems. Most tension wood has a cellulose-rich G-layer in the innermost surface of the fiber cell wall. Strong tensile stress is considered to occur in the G-layer. This study undertook to identify the proteins involved in G-layer formation and function through a proteomic analysis of G-layer-localized protein. G-layers of poplar were loosened by sonication and isolated as doughnut-shaped pieces of thinly sliced transverse sections. The proteins, once extracted with urea/detergent solution, were separated by two-dimensional polyacrylamide gel electrophoresis, and 110 spots were subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS). A database search for these spots' mass spectrum patterns identified 72 proteins. In addition, all peptide digestion mixtures of G-layer proteins were separated by strong cation exchange chromatography and 39 proteins were identified using LC/MS/MS analysis. Proteins involved in wall formation, such as lignin biosynthesis-related protein, xyloglucan endotransglucosylase, and fasciclin-like arabinogalactan protein, were notably detected in the G-layer.
- リンク情報
- ID情報
-
- DOI : 10.1007/s10086-009-1032-6
- ISSN : 1435-0211
- CiNii Articles ID : 10025628364
- Web of Science ID : WOS:000269058800002