【Biodiversity】Development of Next-Generation Sequencing (NGS)-based Technologies for Accurate, Rapid and Cost effective Assessment of Freshwater Species and Genetic Diversities
【Evolution】Adaptive Evolution of Freshwater Biodiversity at Gene and Species Levels Associated with Spatio-temporal Environmental Variation (e.g. Climate Change)
【Eco-Health】 Molecular Genetic Approach for Control of Vector-borne Diseases (e.g. Population Genetics and Virus Surveys of Dengue Mosquito in the Philippines and Indonesia)
【International】 Study Fields Widely Cover Japan, Italian Alps, Swiss, Indonesia and Philippines. Active International Collaboration!
Carvajal, T. M., K. Hashimoto, K. J. D. Go, M. A. I. J. Cruz, M. J. L. B. Martinez, J. D. R. Capistrano, V. S. P. Tiopianco, D. M. Amalin, and K. Watanabe
Journal of Vector Borne Diseases 55 265-270 Dec 2018 [Refereed]
Herbuela, V. R. D. M., F. S. d Guzman, G. D. Sobrepena, A. B. F. Claudio, A. C. V. Tomas, C. M. A-d Reyes, R. A. Regalado, M. M. Teodoro, and K. Watanabe
We have determined nucleotide sequences of three genes encoding histone H3, 18S rRNA, and mitochondrial cytochrome oxidase subunit I (COI) from the mayfly community in Eastern Asia. All sequence data collected from 303 specimens comprising 80 taxa...
Thaddeus M. Carvajal, Lara Fides T. Hernandez, Howell T. Ho, Menard G. Cuenca, Bianca Marie C. Orantia, Camille R. Estrada, Divina M. Amalin, and Kozo Watanabe
Journal of Vector Borne Diseases 53(2) 127-135 Apr 2016 [Refereed]
Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research) 71(7) III_115-III_121 Dec 2015 [Refereed]
We investigated gene flow of Epeorus latifolium in Shigenobu River (Ehime, Japan) fragmented by 3 types of barriers (i.e. one reservoir dam, 98 weir&check dam, intermittent flow). 112 individuals (13 - 15 individuals / population, average = 14) w...
PROCEEDINGS OF ENVIRONMENTAL AND SANITARY ENGINEERING RESEARCH 70(7) III_295-III_304 Dec 2014 [Refereed]
To predict transmission pattern of Norovirus gastroenteritis at regional scale, we've constructed an infectious disease transmission model that considers secondary infection in households. In the model, a household-scale submodel that could simul...
Kozo Watanabe, Kozo Watanabe, So Kazama, Tatsuo Omura, Michael T. Monaghan
PLoS ONE 9(3) e93055 Mar 2014 [Refereed]
A central question linking ecology with evolutionary biology is how environmental heterogeneity can drive adaptive genetic divergence among populations. We examined adaptive divergence of four stream insects from six adjacent catchments in Japan b...
NUKAZAWA Kei, KAZAMA So, TAKASE Akihiko, WATANABE Kozo
PROCEEDINGS OF THE JAPANESE CONFERENCE ON HYDRAULICS 70(4) I_1405-I_1410 Mar 2014 [Refereed]
We evaluated the linkage between genetic diversity and habitat suitability index (HSI) of aquatic animals including freshwater fishes, amphibians and macroinvertebrates in the Natori River basin located at the middle of Miyagi prefecture. The HSI ...
Freshwater Biology 59 1474-1483 Jan 2014 [Refereed]
Summary: The 2011 Great East Japan Earthquake generated extreme tsunami waves that entered river mouths, causing strong and long-distance propagation along the water corridors. This study reports the first empirical survey of this rare and extreme...
Sakiko Yaegashi, Sakiko Yaegashi, Kozo Watanabe, Kozo Watanabe, Michael T. Monaghan, Tatsuo Omura
Freshwater Science 33 172-180 Jan 2014 [Refereed]
Most population genetic studies in streams infer long-term patterns of gene flow by calculating fixation indices (e.g., F ST) among sampled populations. In more-recent analytical methods, the need to assign individuals to populations a priori (clu...
TAKASE Akihiko, NUKAZAWA Kei, KAZAMA So, WATANABE Kozo
PROCEEDINGS OF THE JAPANESE CONFERENCE ON HYDRAULICS 70(4) I_1297-I_1302 2014 [Refereed]
This study quantitatively evaluated Habitat Suitability Index (HSI) and species diversity of benthic invertebrates using a distributed hydro-thermal model and observed benthic samples from 45 localities in the Natori River basin, Northeast of Japa...
PROCEEDINGS OF ENVIRONMENTAL AND SANITARY ENGINEERING RESEARCH 69(7) III_489-III_494 Dec 2013 [Refereed]
Environmental heterogeneity among habitats may be one of the factors causing adaptive genetic divergence in wild populations. We examined the effect of natural selection on genetic diversity of Stenopsyche marmorata population in middle of...
PROCEEDINGS OF ENVIRONMENTAL AND SANITARY ENGINEERING RESEARCH 69(7) III_547-III_555 Nov 2013 [Refereed]
Dams often supply large amount of Fine Particulate Organic Matters (FPOM) orginated from dam reservoirs (called "dam-POM") to their downstream reaches. It potentially leads to a shift of trophic sources of stream faunal communities from the strea...
TAKASE Akihiko, NUKAZAWA Kei, KAZAMA So, WATANABE Kozo
PROCEEDINGS OF THE JAPANESE CONFERENCE ON HYDRAULICS 69(4) I_1255-I_1260 2013 [Refereed]
We evaluated spatial variations of habitat suitability of nine aquatic insects in the Natori River basin, Northeast Japan quantitatively combining dual approaches of using Habitat Suitability Index (HSI) model analysis and hydrothermal simulation ...
PROCEEDINGS OF THE JAPANESE CONFERENCE ON HYDRAULICS 69(4) I_1303-I_1308 2013 [Refereed]
In accordance with a most recent report in discipline "landscape genetics", simulation studies to predict spatial genetic variation are urgently required. We evaluated relationships between genetic diversities of caddisfly Hydropsyche orientalis a...
PROCEEDINGS OF ENVIRONMENTAL AND SANITARY ENGINEERING RESEARCH 68(7) III_603-III_610 Nov 2012 [Refereed]
Relationships between species diversity calculated from Habitat Suitability Index (HSI) of aquatic animalms and genetic diversities of four species of aquatic insect (three caddisflies and one mayfly) were examined in the Natori River basin, midd...
Ecology and Civil Engineering 15 121-130 Oct 2012 [Refereed]
Spatial structures of 7 types of aquatic habitat at the reach scale were delineated using a high precision Global Position System (GPS) in 3 dam-impacted and 6 reference riverrine reaches in Miyagi Prefecture, Japan. Taxonomic and functional compo...
PROCEEDINGS OF ENVIRONMENTAL AND SANITARY ENGINEERING RESEARCH 68(7) III_611-III_616 2012 [Refereed]
Spatial environmental heterogeneity may favorably sustain genetic diversity of wild populations through adaptive divergence. We empirically examined the relationship between habitat structure and genetic diversity of stream mayfly Ephemera str...
WIT Transactions on Ecology and the Environment 146 263-274 Dec 2011 [Refereed]
In order to assess the impacts of sabo dam (check dam) construction, species diversity of benthic faunal communities and genetic diversity of caddisfly Stenopsyche marmorata were investigated using microsatellite analysis along the Oisawa River, J...
1. The dispersal ability of species and the geographic scale of habitat fragmentation both may influence the extent of gene flow between fragments, but their interactions have rarely been tested, particularly among co-occurring species. 2. Populat...
This article documents the addition of 283 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Agalinis acuta; Ambrosia artemisiifolia; Berula erecta; Casuarius casuarius; Cercospo...
Yoshiyuki Shirotori, Kozo Watanabe, Keiko Udo, Akira Mano
Advances in Water Resources and Hydraulic Engineering - Proceedings of 16th IAHR-APD Congress and 3rd Symposium of IAHR-ISHS 815-820 Jan 2009 [Refereed]
On the upstream of Atago weir, Hirose River, Japan, the sedimentation phenomenon is complex due to the coexistence of the weir and channel bend. Moreover, fine sediment accumulates on the river bed notably not only the floodplain. In this study, w...
Kozo Watanabe, Michael T. Monaghan, Michael T. Monaghan, Michael T. Monaghan, Tatsuo Omura
Aquatic Sciences 70 377-387 Dec 2008 [Refereed]
Small local populations may have low levels of genetic diversity, although widespread gene flow may counteract genetic drift and maintain high local diversity. At a larger spatial scale, a relationship between population size and genetic diversity...
Water Science and Technology 58(3) 503-512 Oct 2008 [Refereed]
The trophic structure of a macroinvertebrate community was investigated using carbon and nitrogen stable isotopes analysis (δ13c and δ15N) at three longitudinal sites along the main channel of the Hirose River, Japan, and at an outlet site of the ...
Kozo Watanabe, Michael T. Monaghan, Michael T. Monaghan, Yasuhiro Takemon, Tatsuo Omura
Science of the Total Environment 394(1) 57-67 May 2008 [Refereed]
Analysis of carbon (δ13C) and nitrogen (δ15N) stable isotopes provides an increasingly important means of understanding the complex trophic structure of macroinvertebrate communities in streams. We coupled a stable isotope approach with a contamin...
Reservoirs have the potential to block the gene flow of stream macroinvertebrates along a channel, which takes place via larval drift and adult flight, resulting in genetic differentiation above and below the reservoirs. Gene flow across the reser...
Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research) 62(1) 94-104 Apr 2006 [Refereed]
Based on RAPD, the genetic structures of Stenopsyche marmorata, Hydropsyche orientalis, and Ephemerella nigra populations found above and below reservoirs with various water surface areas were investigated. The results of the genetic...
Ecological Modelling 189 396-412 Dec 2005 [Refereed]
A macroinvertebrate community having been pre-assessed to have lower resilience against anthropogenic pulse-disturbance in river (e.g. muddy water from construction site) should be given intensive preservation efforts. Using stochastic population ...
Constructed wetlands (CWs) for wastewater treatment had drawn much attention for their additional function of conserving freshwater ecosystems. The purpose of this study was to evaluate the macroinvertebrate biodiversity of two subsurface-flow and...
Health in Ecological Perspectives in the Anthropocene
Carvajal, T. M., H. T. Ho, L. F. T. Hernandez, K. M. Viacrusis, D. M. Amalin, and K. Watanabe (Part:Joint Work, An Ecological Context towards Understanding Dengue Disease Dynamics in Urban Cities: a Case Study in Metropolitan Manila, Philippines)
cee.ehime-u.ac.jp