2013年
Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.
PloS one
- ,
- 巻
- 8
- 号
- 7
- 開始ページ
- e68671
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0068671
Lipopolysaccharide (LPS) is responsible for many of the inflammatory responses and pathogenic effects of Gram-negative bacteria, however, it also induces protective immune responses. LPS induces the production of inflammatory cytokines such as TNF-α, IL-6, and IL-12 from dendritic cells (DCs) and macrophages. It is thought that IL-12 is required for one of the protective immune responses induced by LPS, the T helper 1 (Th1)-immune response, which include the production of IFN-γ from Th1cells and IgG2c class switching. Here, we clearly demonstrate that intracellular delivery of LPS by LPS-formulated liposomes (LPS-liposomes) does not induce the production of inflammatory cytokines from DCs, but enhances Th1-immune responses via type-I IFNs, independent of IL-12. Collectively, our results strongly suggest that LPS-liposomes can effectively induce Th1-immune responses without inducing unnecessary inflammation, and may be useful as an immune adjuvant to induce protective immunity.
- リンク情報
- ID情報
-
- DOI : 10.1371/journal.pone.0068671
- ISSN : 1932-6203
- PubMed ID : 23874715
- PubMed Central 記事ID : PMC3714268
- SCOPUS ID : 84880396463