2008年10月
Directed aggregation and fusion of lipid vesicles induced by DNA-surfactants
COLLOIDS AND SURFACES B-BIOINTERFACES
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- 巻
- 66
- 号
- 1
- 開始ページ
- 119
- 終了ページ
- 124
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.colsurfb.2008.05.017
- 出版者・発行元
- ELSEVIER SCIENCE BV
We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested char the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle. (c) 2008 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.colsurfb.2008.05.017
- ISSN : 0927-7765
- PubMed ID : 18617375
- Web of Science ID : WOS:000258983900018