2020年11月18日
Optimization of Wide-Field ODMR Measurements Using Fluorescent Nanodiamonds to Improve Temperature Determination Accuracy
Nanomaterials
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- 巻
- 10
- 号
- 11
- 開始ページ
- 2282
- 終了ページ
- 2282
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/nano10112282
- 出版者・発行元
- MDPI AG
Fluorescent nanodiamonds containing nitrogen-vacancy centers have attracted attention as nanoprobes for temperature measurements in microenvironments, potentially enabling the measurement of intracellular temperature distributions and temporal changes. However, to date, the time resolution and accuracy of the temperature determinations using fluorescent nanodiamonds have been insufficient for wide-field fluorescence imaging. Here, we describe a method for highly accurate wide-field temperature imaging using fluorescent nanodiamonds for optically detected magnetic resonance (ODMR) measurements. We performed a Monte Carlo simulation to determine the optimal frequency sweep range for ODMR temperature determination. We then applied this sweep range to fluorescent nanodiamonds. As a result, the temperature determination accuracies were improved by a factor ~1.5. Our result paves the way for the contribution of quantum sensors to cell biology for understanding, for example, differentiation in multicellular systems.
- リンク情報
- ID情報
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- DOI : 10.3390/nano10112282
- eISSN : 2079-4991
- SCOPUS ID : 85096209359