論文

査読有り
2017年1月1日

Phosphorus remobilization from rice flag leaves during grain filling: an RNA-seq study

Plant Biotechnology Journal
  • Kwanho Jeong
  • ,
  • Abdul Baten
  • ,
  • Daniel L. E. Waters
  • ,
  • Omar Pantoja
  • ,
  • Cecile C. Julia
  • ,
  • Matthias Wissuwa
  • ,
  • Sigrid Heuer
  • ,
  • Tobias Kretzschmar
  • ,
  • Terry J. Rose

15
1
開始ページ
15
終了ページ
26
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/pbi.12586
出版者・発行元
Blackwell Publishing Ltd

The physiology and molecular regulation of phosphorus (P) remobilization from vegetative tissues to grains during grain filling is poorly understood, despite the pivotal role it plays in the global P cycle. To test the hypothesis that a subset of genes involved in the P starvation response are involved in remobilization of P from flag leaves to developing grains, we conducted an RNA-seq analysis of rice flag leaves during the preremobilization phase (6 DAA) and when the leaves were acting as a P source (15 DAA). Several genes that respond to phosphate starvation, including three purple acid phosphatases (OsPAP3, OsPAP9b and OsPAP10a), were significantly up-regulated at 15 DAA, consistent with a role in remobilization of P from flag leaves during grain filling. A number of genes that have not been implicated in the phosphate starvation response, OsPAP26, SPX-MFS1 (a putative P transporter) and SPX-MFS2, also showed expression profiles consistent with involvement in P remobilization from senescing flag leaves. Metabolic pathway analysis using the KEGG system suggested plastid membrane lipid synthesis is a critical process during the P remobilization phase. In particular, the up-regulation of OsPLDz2 and OsSQD2 at 15 DAA suggested phospholipids were being degraded and replaced by other lipids to enable continued cellular function while liberating P for export to developing grains. Three genes associated with RNA degradation that have not previously been implicated in the P starvation response also showed expression profiles consistent with a role in P mobilization from senescing flag leaves.

リンク情報
DOI
https://doi.org/10.1111/pbi.12586
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27228336
ID情報
  • DOI : 10.1111/pbi.12586
  • ISSN : 1467-7652
  • ISSN : 1467-7644
  • ORCIDのPut Code : 75770972
  • PubMed ID : 27228336
  • SCOPUS ID : 84977083481

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