論文

査読有り 国際誌
2017年9月1日

Fast voltage-sensitive dye imaging of excitatory and inhibitory synaptic transmission in the rat granular retrosplenial cortex.

Journal of neurophysiology
  • Ken'ichi Nixima
  • ,
  • Kazuo Okanoya
  • ,
  • Noritaka Ichinohe
  • ,
  • Tohru Kurotani

118
3
開始ページ
1784
終了ページ
1799
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1152/jn.00734.2016

Rodent granular retrosplenial cortex (GRS) has dense connections between the anterior thalamic nuclei (ATN) and hippocampal formation. GRS superficial pyramidal neurons exhibit distinctive late spiking (LS) firing property and form patchy clusters with prominent apical dendritic bundles. The aim of this study was to investigate spatiotemporal dynamics of signal transduction in the GRS induced by ATN afferent stimulation by using fast voltage-sensitive dye imaging in rat brain slices. In coronal slices, layer 1a stimulation, which presumably activated thalamic fibers, evoked propagation of excitatory synaptic signals from layers 2-4 to layers 5-6 in a direction perpendicular to the layer axis, followed by transverse signal propagation within each layer. In the presence of ionotropic glutamate receptor antagonists, inhibitory responses were observed in superficial layers, induced by direct activation of inhibitory interneurons in layer 1. In horizontal slices, excitatory signals in deep layers propagated transversely mainly from posterior to anterior via superficial layers. Cortical inhibitory responses upon layer 1a stimulation in horizontal slices were weaker than those in the coronal slices. Observed differences between coronal and horizontal planes suggest anisotropy of the intracortical circuitry. In conclusion, ATN inputs are processed differently in coronal and horizontal planes of the GRS and then conveyed to other cortical areas. In both planes, GRS superficial layers play an important role in signal propagation, which suggests that superficial neuronal cascade is crucial in the integration of multiple information sources.NEW & NOTEWORTHY Superficial neurons in the rat granular retrosplenial cortex (GRS) show distinctive late-spiking (LS) firing property. However, little is known about spatiotemporal dynamics of signal transduction in the GRS. We demonstrated LS neuron network relaying thalamic inputs to deep layers and anisotropic distribution of inhibition between coronal and horizontal planes. Since deep layers of the GRS receive inputs from the subiculum, GRS circuits may work as an integrator of multiple sources such as sensory and memory information.

リンク情報
DOI
https://doi.org/10.1152/jn.00734.2016
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28701546
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596128
ID情報
  • DOI : 10.1152/jn.00734.2016
  • PubMed ID : 28701546
  • PubMed Central 記事ID : PMC5596128

エクスポート
BibTeX RIS