2015年5月
Nucleotides maintain the activity of Cav1.2 channels in guinea-pig ventricular myocytes
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- 巻
- 460
- 号
- 3
- 開始ページ
- 813
- 終了ページ
- 818
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bbrc.2015.03.111
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
The activity of Cav1.2 Ca2+ channels is maintained in the presence of calmodulin and ATP, even in cell-free patches, and thus a channel ATP-binding site has been suggested. In this study, we examined whether other nucleotides, such as GTP, UTP, CTP, ADP and AMP, could be substituted for ATP in guinea-pig ventricular myocytes. We found that all the nucleotides tested could re-prime the Ca2+ channels in the presence of 1 mu M calmodulin in the inside-out mode. The order of efficacy was ATP > GTP > UTP > ADP > CTP AMP. Thus, the presumed nucleotide-binding site in the channel seemed to favor a purine rather than pyrimidine base and a triphosphate rather than a di- or monophosphate group. Furthermore, a high concentration (10 mM) of GTP, UTP, CTP, ADP and AMP had inhibitory effects on the channel activity. These results provide information on the putative nucleotide-binding site(s) in Cav1.2 Ca2+ channels. (C) 2015 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bbrc.2015.03.111
- ISSN : 0006-291X
- eISSN : 1090-2104
- Web of Science ID : WOS:000359885300052