論文

査読有り
2014年10月

Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

SCIENCE
  • Bi-Chang Chen
  • Wesley R. Legant
  • Kai Wang
  • Lin Shao
  • Daniel E. Milkie
  • Michael W. Davidson
  • Chris Janetopoulos
  • Xufeng S. Wu
  • John A. Hammer
  • Zhe Liu
  • Brian P. English
  • Yuko Mimori-Kiyosue
  • Daniel P. Romero
  • Alex T. Ritter
  • Jennifer Lippincott-Schwartz
  • Lillian Fritz-Laylin
  • R. Dyche Mullins
  • Diana M. Mitchell
  • Joshua N. Bembenek
  • Anne-Cecile Reymann
  • Ralph Boehme
  • Stephan W. Grill
  • Jennifer T. Wang
  • Geraldine Seydoux
  • U. Serdar Tulu
  • Daniel P. Kiehart
  • Eric Betzig
  • 全て表示

346
6208
開始ページ
439
終了ページ
+
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1126/science.1257998
出版者・発行元
AMER ASSOC ADVANCEMENT SCIENCE

Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools. We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.

リンク情報
DOI
https://doi.org/10.1126/science.1257998
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25342811
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000349773700002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1126/science.1257998
  • ISSN : 0036-8075
  • eISSN : 1095-9203
  • PubMed ID : 25342811
  • Web of Science ID : WOS:000349773700002

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