In this review, we focus on the progress of temperature-responsive cell culture surface (TRCS), which is a poly(N-isopropylacrylamide) (PIPAAm) grafted surface, since the first report of temperature-responsive surfaces. We also show that the optimization of the grafted nano-scaled PIPAAm layer is essential to express temperature dependent cell attachment/detachment. Also, we describe the technologies required to readily fabricate cell sheets and TRCS in the near future, which are quantitative methods for cell adhesion/detachment processes from TRCS by using micro fluidic technique and a facile method for preparation of TRCS.