論文

査読有り
2017年2月

Prevention of Vocal Fold Scarring by Local Application of Basic Fibroblast Growth Factor in a Rat Vocal Fold Injury Model

LARYNGOSCOPE
  • Ryo Suzuki
  • ,
  • Yoshitaka Kawai
  • ,
  • Takuya Tsuji
  • ,
  • Nao Hiwatashi
  • ,
  • Yo Kishimoto
  • ,
  • Ichiro Tateya
  • ,
  • Tatsuo Nakamura
  • ,
  • Shigeru Hirano

127
2
開始ページ
E67
終了ページ
E74
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/lary.26138
出版者・発行元
WILEY

Objectives/Hypothesis: Vocal fold scarring, which causes severe hoarseness, is intractable. The optimal treatment for vocal fold scarring has not been established; therefore, prevention of scarring is important. The aim of this study was to clarify the effectiveness of basic fibroblast growth factor ( bFGF) for prevention of postsurgical vocal fold scarring.
Study Design: Prospective animal experiments with controls.
Method: The vocal folds of Sprague-Dawley rats were injured unilaterally or bilaterally after local application of a 10 mu L solution of bFGF. Larynges ware harvested for histological and immunohistochemical examination 2 months postoperation and for quantitative real-time polymerase chain reaction ( qRT-PCR) analysis 1 week postoperation.
Results: Histological examination showed significantly increased hyaluronic acid and decreased deposition of dense collagen in the bFGF-treated group at 100 ng/10 mu L compared with the sham-treated group. Immunohistochemical examination showed significantly decreased collagen type III deposition in the bFGF-treated group at 100 ng/10 mu L compared with the sham-treated group. qRT-PCR revealed that hyaluronan synthase 2 ( Has2), Has3, and hepatocyte growth factor were upregulated in bFGF-treated groups compared with sham-treated group.
Conclusion: The current results suggest that local application of bFGF at the time of injury has the potential to prevent vocal fold scarring. Preventive injection of bFGF could be applied at the time of phonomicrosurgery to avoid postoperative scar formation.

Web of Science ® 被引用回数 : 16

リンク情報
DOI
https://doi.org/10.1002/lary.26138
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000394962600003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/lary.26138
  • ISSN : 0023-852X
  • eISSN : 1531-4995
  • Web of Science ID : WOS:000394962600003

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