論文

査読有り
2017年5月

The efficacy of a novel collagen-gelatin scaffold with basic fibroblast growth factor for the treatment of vocal fold scar

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
  • Nao Hiwatashi
  • ,
  • Shigeru Hirano
  • ,
  • Masanobu Mizuta
  • ,
  • Toshiki Kobayashi
  • ,
  • Yoshitaka Kawai
  • ,
  • Shin-ichi Kanemaru
  • ,
  • Tatsuo Nakamura
  • ,
  • Juichi Ito
  • ,
  • Katsuya Kawai
  • ,
  • Shigehiko Suzuki

11
5
開始ページ
1598
終了ページ
1609
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/term.2060
出版者・発行元
WILEY

Vocal fold scar remains a therapeutic challenge. Basic fibroblast growth factor (bFGF) was reported to have regenerative effects for vocal fold scar, although it has the disadvantage of rapid absorption in vivo. A collagen-gelatin sponge (CGS) can compensate for the disadvantage by providing a sustained release system. The current study evaluated the efficacy of CGS combined with bFGF on vocal fold scar, using rat fibroblasts for an in vitro model and a canine in vivo model. We prepared fibroblasts from scarred vocal folds (sVFs) in rats and showed that bFGF accelerated cell proliferation and suppressed expression levels of cleaved caspase 3 and alpha-smooth muscle actin. Has 1, Has 3, Fgf2, Hgf and Vegfa mRNA levels were significantly upregulated, while Col1a1 and Col3a1 were dose-dependently downregulated, with a maximum effect at 100 ng/ml bFGF. In an in vivo assay, 6 weeks after lamina propria stripping, beagles were divided into three groups: CGS alone (CGS group); CGS with bFGF (7 mu g/cm(2); CGS + bFGF group); or a sham-treated group. Vibratory examination revealed that the glottal gap was significantly reduced in the bFGF group and the two implanted groups, whereas the CGS + bFGF group showed higher mucosal wave amplitude. Histological examination revealed significantly restored hyaluronic acid and elastin redistribution in the CGS + bFGF group and reductions in dense collagen deposition. These results provide evidence that CGS and bFGF combination therapy may have therapeutic potential and could be a promising tool for treating vocal fold scar. Copyright (C) 2015 John Wiley & Sons, Ltd.

Web of Science ® 被引用回数 : 15

リンク情報
DOI
https://doi.org/10.1002/term.2060
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000402987500026&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/term.2060
  • ISSN : 1932-6254
  • eISSN : 1932-7005
  • Web of Science ID : WOS:000402987500026

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