© 2018 The Institute of Electrical Engineers of Japan. This paper considers a functional electrical stimulation (FES) cycling with cadence tracking control for rehabilitation of hemiparesis. A recumbent trike and rider with four muscle groups is modeled as an Euler Lagrange system based on a pedaling force direction. We propose cadence tracking control considering effects of antagonistic bi-articular muscles to stimulate multiple muscle groups continuously at all angles during pedaling motion. Stability of the developed controller is analyzed through Lyapunov-based methods. Experimental results in healthy participants are shown to confirm the validity of the proposed method and the potential for the rehabilitation of hemiparesis patients.