Yasuhisa Kimura

Profile Information

Affiliation: 准教授, 農学研究科, 京都大学

Degree: 博士(農学)(Jul, 2005, 京都大学)

J-GLOBAL ID: 201801021067306975
researchmap Member ID: B000326409

Research Interests

Research Areas

Life sciences / Applied biochemistry / cellular biochemistry

Research History

Nov, 2020 - Present

Graduate School of Agriculture, Kyoto University
Jan, 2007 - Oct, 2020

助教, 農学研究科, 京都大学大学院
Apr, 2006 - Dec, 2006

研究員, 薬学研究科, 京都大学大学院
Apr, 2005 - Mar, 2006

研究員, 播磨研究所, 理化学研究所

Education

Apr, 2002 - Mar, 2005

応用生命科学専攻博士後期課程, 農学研究科, 京都大学大学院
Apr, 2000 - Mar, 2002

応用生命科学専攻博士前期課程, 農学研究科, 京都大学大学院
Apr, 1996 - Mar, 2000

Faculty of Agriculture, Kyoto University

Papers

The ATPase activity of ABCA1 is increased by cholesterol in the presence of anionic lipids

Kazuki Sakata, Noriyuki Kioka, Kazumitsu Ueda, Yasuhisa Kimura

The Journal of Biochemistry, May 31, 2024
The plasma membrane of focal adhesions has a high content of cholesterol and phosphatidylcholine with saturated acyl chains.

Hiroshi Tachibana, Kodai Minoura, Tomohiro Omachi, Kohjiro Nagao, Takafumi Ichikawa, Yasuhisa Kimura, Nozomu Kono, Yuta Shimanaka, Hiroyuki Arai, Kazumitsu Ueda …

Journal of cell science, 136(16), Aug 15, 2023
ABCB1/MDR1/P-gp employs an ATP-dependent twist-and-squeeze mechanism to export hydrophobic drugs.

Atsushi Kodan, Ryota Futamata, Yasuhisa Kimura, Noriyuki Kioka, Toru Nakatsu, Hiroaki Kato, Kazumitsu Ueda

FEBS letters, 595(6) 707-716, Mar, 2021
ABCA13 dysfunction associated with psychiatric disorders causes impaired cholesterol trafficking

Mitsuhiro Nakato, Naoko Shiranaga, Maiko Tomioka, Hitomi Watanabe, Junko Kurisu, Mineko Kengaku, Naoko Komura, Hiromune Ando, Yasuhisa Kimura, Noriyuki Kioka …

Journal of Biological Chemistry, 296 100166-100166, 2021
Stiffness of the extracellular matrix regulates differentiation into beige adipocytes.

Kyoko Takata, Tsuyoshi Goto, Mito Kuroda, Yasuhisa Kimura, Ichiro Harada, Kazumitsu Ueda, Teruo Kawada, Noriyuki Kioka

Biochemical and biophysical research communications, 532(2) 205-210, Nov 5, 2020
In vivo FRET analyses reveal a role of ATP hydrolysis–associated conformational changes in human P-glycoprotein

Ryota Futamata, Fumihiko Ogasawara, Takafumi Ichikawa, Atsushi Kodan, Yasuhisa Kimura, Noriyuki Kioka, Kazumitsu Ueda

Journal of Biological Chemistry, 295(15) 5002-5011, Apr 10, 2020 Peer-reviewed
Inward- and outward-facing X-ray crystal structures of homodimeric P-glycoprotein CmABCB1

Atsushi Kodan, Tomohiro Yamaguchi, Toru Nakatsu, Keita Matsuoka, Yasuhisa Kimura, Kazumitsu Ueda, Hiroaki Kato

Nature Communications, 10(1) 88-88, Dec 1, 2019
Changes in the asymmetric distribution of cholesterol in the plasma membrane influence streptolysin O pore formation

Fumihiko Ogasawara, Fumi Kano, Masayuki Murata, Yasuhisa Kimura, Noriyuki Kioka, Kazumitsu Ueda

Scientific Reports, 9, Dec 1, 2019
C-terminal of ABCA1 separately regulates cholesterol floppase activity and cholesterol efflux activity.

Okamoto Y, Tomioka M, Ogasawara F, Nagaiwa K, Kimura Y, Kioka N, Ueda K

Bioscience, biotechnology, and biochemistry, 1-10, Dec, 2019 Peer-reviewed
The role of ATP hydrolysis in conformational changes of human P-glycoprotein in living cells

Ryota Futamata, Fumihiko Ogasawara, Takafumi Ichikawa, Atsushi Kodan, Yasuhisa Kimura, Noriyuki Kioka, Kazumitsu Ueda

bioRxiv, Nov, 2019
How ATP is used by P-glycoprotein to export multiple hydrophobic compounds

Ryota Futamata, Fumihiko Ogasawara, Takafumi Ichikawa, Yasuhisa Kimura, Noriyuki Kioka, Kazumitsu Ueda

May, 2019
An amphipathic helix of vinexin α is necessary for a substrate stiffness-dependent conformational change in vinculin

Naoya Hino, Takafumi Ichikawa, Yasuhisa Kimura, Michiyuki Matsuda, Kazumitsu Ueda, Noriyuki Kioka

Journal of Cell Science, 132(2), Jan 1, 2019
Apolipoprotein A-I directly interacts with extracellular domain 1 of human ABCA1

Takaaki Kawanobe, Naoko Shiranaga, Noriyuki Kioka, Yasuhisa Kimura, Kazumitsu Ueda

Bioscience, Biotechnology and Biochemistry, 83 490-497, Jan 1, 2019
Estimation of ABCB1 concentration in plasma membrane

Petr Mlejnek, Petr Kosztyu, Petr Dolezel, Yasuhisa Kimura, Katerina Cizkova, Eliska Ruzickova

Journal of Cellular Biochemistry, 120 18406-18414, Jan 1, 2019
Temporary sequestration of cholesterol and phosphatidylcholine within extracellular domains of ABCA1 during nascent HDL generation

Masato Ishigami, Fumihiko Ogasawara, Kohjiro Nagao, Hidehiko Hashimoto, Yasuhisa Kimura, Noriyuki Kioka, Kazumitsu Ueda

Scientific Reports, 8(1), Dec 1, 2018
Leishmania LABCG2 transporter is involved in ATP-dependent transport of thiols

Ana Perea, José Ignacio Manzano, Yasuhisa Kimura, Kazumitsu Ueda, Santiago Castanys, Francisco Gamarro

Biochemical Journal, 475(1) 87-97, Jan 15, 2018
Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation

Takafumi Ichikawa, Masahiro Kita, Tsubasa S. Matsui, Ayaka Ichikawa Nagasato, Tomohiko Araki, Shian-Huey Chiang, Takuhito Sezaki, Yasuhisa Kimura, Kazumitsu Ueda, Shinji Deguchi …

JOURNAL OF CELL SCIENCE, 130(20) 3517-3531, Oct, 2017
Vinculin association with actin cytoskeleton is necessary for stiffness-dependent regulation of vinculin behaviore

Tomohiro Omachi, Takafumi Ichikawa, Yasuhisa Kimura, Kazumitsu Ueda, Noriyuki Kioka

PLOS ONE, 12(4), Apr, 2017
Vinculin promotes nuclear localization of TAZ to inhibit ECM stiffness-dependent differentiation into adipocytes

Mito Kuroda, Hiroki Wada, Yasuhisa Kimura, Kazumitsu Ueda, Noriyuki Kioka

JOURNAL OF CELL SCIENCE, 130(5) 989-1002, Mar, 2017
Structure-activity relationships of dibenzoylhydrazines for the inhibition of P-glycoprotein-mediated quinidine transport

Ken-ichi Miyata, Yoshiaki Nakagawa, Yasuhisa Kimura, Kazumitsu Ueda, Miki Akamatsu

BIOORGANIC & MEDICINAL CHEMISTRY, 24(14) 3184-3191, Jul, 2016

Misc.

174

A system for transient localization of lipid-modifying enzyme to focal adhesion using chemically induced dimerization

増元亮裕, 木村泰久, 木岡紀幸

日本細胞生物学会大会(Web), 73rd, 2021
FA membrane has a specific lipid composition

立花大, 長尾耕治郎, 木村泰久, 木岡紀幸

日本細胞生物学会大会(Web), 73rd, 2021
Purification of Focal Adhesion Protein, Vinexin α using Mammalian Cells

鎌田一希, 木村泰久, 木岡紀幸

日本農芸化学会大会講演要旨集(Web), 2021, 2021
C-terminus of ABCA1 separately regulates cholesterol-flopping activity and HDL-generating activity

小笠原史彦, 岡本雄介, 木村泰久, 木岡紀幸, 木岡紀幸, 植田和光

日本農芸化学会大会講演要旨集(Web), 2021, 2021
A system for transient PIP5K localization to focal adhesion using chemically induced dimerization

増元亮裕, 木村泰久, 木岡紀幸

日本農芸化学会大会講演要旨集(Web), 2021, 2021
Expression and purification of a lipid efflux ABC protein aiming for Cryo-EM analysis

小段篤史, 光岡薫, 木村泰久, 木岡紀幸, 植田和光

日本農芸化学会大会講演要旨集(Web), 2021, 2021
SORBS family proteins regulate meflin expression

高橋真助, 柴原正和, 榎本篤, 木村泰久, 木岡紀幸

日本農芸化学会大会講演要旨集(Web), 2021, 2021
Thermodynamic analysis of the enhancement of substrate-dependent ATPase activity of MDR1 by cholesterol.

岩村紗花, 木岡紀幸, 植田和光, 木村泰久

日本農芸化学会大会講演要旨集(Web), 2021, 2021
The identification of new focal adhesion proteins that are related with mechanosensing

田辺修平, 柴原正和, 立花大, 木村泰久, 木岡紀幸

日本農芸化学会大会講演要旨集(Web), 2021, 2021
The effect of collagen peptide on proliferation and migration of cancer cells

倉田淳代, 木村泰久, 松尾道憲, 木岡紀幸

日本農芸化学会大会講演要旨集(Web), 2021, 2021
The analysis of the sterol specificity of human ABCA1 using liposomes

坂田和樹, 木岡紀幸, 植田和光, 木村泰久

日本農芸化学会大会講演要旨集(Web), 2021, 2021
Comparative analysis of the lipid composition of focal adhesion membrane and plasma membrane

立花大, 箕浦広大, 長尾耕治郎, 木村泰久, 植田和光, 木岡紀幸

日本農芸化学会大会講演要旨集(Web), 2021, 2021
ヒトABCA1の活性誘導とステロール構造の関連解析

坂田和樹, 木岡紀幸, 植田和光, 木村泰久

脂質生化学研究, 63, 2021
The effect of collagen peptide on cancer cell

倉田淳代, 木村泰久, 松尾道憲, 木岡紀幸

日本農芸化学会大会講演要旨集(Web), 2020, 2020
コレステロールによるMDR1の活性誘導とrhodamine化合物の構造関連性

岩村紗花, 木岡紀幸, 植田和光, 木村泰久

日本生化学会大会(Web), 93rd, 2020
ABCA1のHDL産生活性およびコレステロールフロップ活性の調節

岡本雄介, 小笠原史彦, 木村泰久, 木岡紀幸, 木岡紀幸, 植田和光

脂質生化学研究, 62, 2020
HPLC-ELSDによるABCA1,ABCB4の脂質排出活性測定

高橋智, 北悠人, 木岡紀幸, 木岡紀幸, 植田和光, 木村泰久

トランスポーター研究会年会抄録集, 15th, 2020
接着斑タンパク質ビネキシンαは相分離に関与するか?

鎌田一希, 木村泰久, 木岡紀幸

日本生化学会大会(Web), 93rd, 2020
コレステロール輸送体ABCA1の生理的役割のメダカを用いた解析

二股良太, 木下政人, 木村泰久, 木岡紀幸, 木岡紀幸, 植田和光

脂質生化学研究, 62, 2020
巨大リポソーム(GUV)を用いたヒトABCA1の新規機能解析手法の開発

坂田和樹, 木岡紀幸, 植田和光, 木村泰久

脂質生化学研究, 62, 2020

Presentations

Functional relationships between lipids and ABC proteins

Yasuhisa Kimura

Sep 15, 2020 Invited
生化学的手法を基軸としたヒトABCタンパク質の機能解析

木村泰久

第92回日本生化学会大会, Sep 18, 2019 Invited
ApoA-I binds to the extracellular domain 1 of human ABCA1

Yasuhisa Kimura, Takaaki Kawanobe, Naoko Shiranaga, Noriyuki Kioka, Kazumitsu Ueda

60th International Conference on the Bioscience of Lipids（ICBL), Jun 18, 2019
ヒト由来浮遊培養細胞への遺伝子導入手法の検討

木村泰久, 松尾道憲, 木岡紀幸, 植田和光

2018年度日本農芸化学会関西支部大会(第505回講演会), Sep 14, 2018
Substrate entry mechanism of human MDR1

Yasuhisa Kimura, Shuichi Inoue, Noriyuki Kioka, Kazumitsu Ueda

7th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases, Mar 6, 2018
ヌクレオチド依存的なKATP チャネル複合体の構造変化

木村泰久

第1 回イオンチャネル研究会, Jul 7, 2016 Invited
Expression and purification of mammalian membrane protein complex from human cultured cells

KIMURA Yasuhisa

Jun 7, 2016 Invited
ヌクレオチド依存的なATP感受性カリウムチャネルの構造変化

木村泰久, 菅野亮, 松尾道憲, 加藤博章, 光岡薫, 植田和光

2016年(平成28年度)日本農芸化学会大会, Mar 27, 2016
Nucleotide induces conformational change in the channel subunit of KATP channel

Yasuhisa Kimura, Ryo Kanno, Michinori Matsuo, Hiroaki Kato, Kaoru Mitsuoka, Kazumitsu Ueda

6th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”, Mar 5, 2016
Analysis of mechanism for stabilizing inward open conformation of human MDR1

Tomomi Tsuyama, Yasuhisa Kimura, Noriyuki Kioka, Hiraoki Kato, Kaztumitsu Ueda

日本農芸化学会2015年度大会, 2015
細胞外マトリクスの硬さ感知におけるビンキュリンのアクチン繊維結合能の重要性

Tomohiro Omachi, Takafumi Ichikawa, Yasuhisa Kimura, Kazumitsu Ueda, Noriyuki Kioka

日本農芸化学会2015年度大会, 2015
Analysis of transport substrates of human ABCA1 and ABCA7 based on ATPase assay

Hidehiko Hashimoto, Masato Ishigami, Yasuhisa Kimura, Kaztumitsu Ueda

日本農芸化学会2015年度大会, 2015
HDL産生時にABCA1は輸送したコレステロールを一時的に細胞外ドメイン内に蓄積する

Masato Ishigami, Kohjiro Nagao, Yasuhisa Kimura, Noriyuki Kioka, Kazumitsu Ueda

日本農芸化学会関西支部例会第488回講演会, 2015
Analysis of cholesterol recognition mechanism of ABCA1 by amino acid substitution

Fumihiko Ogasawara, Yasuhisa Kimura, Kaztumitsu Ueda

日本農芸化学会2015年度大会, 2015
An amphipathic helix in vinexin α interacts with vinculin

Naoya Hino, Takafumi Ichikawa, Yasuhisa Kimura, Kazumitsu Ueda, Noriyuki Kioka

日本農芸化学会2015年度大会, 2015
Identification of a novel natural product fomiroid A, which inhibits NPC1L1-dependent cholesterol uptake, from the mushroom Fomitopsis nigra.

Tomohiro CHIBA, Tsuyoshi SAKURADA, Rie WATANABE, Kohji YAMAGUCHI, Yasuhisa KIMURA, Noriyuki KIOKA, Hirokazu KAWAGISHI, Michinori MATSUO, Kazumitsu UEDA

日本農芸化学会2015年度大会, 2015
Analysis of the substrates entry gate mechanism of human MDR1

Kento Suenaga, Syuichi Inoue, Yasuhisa Kimura, Noriyuki Kioka, Hiraoki Kato, Kaztumitsu Ueda

日本農芸化学会2015年度大会, 2015
軟骨細胞分化における接着斑タンパク質ビネキシンの役割

Yuki Wantani, Yasuhisa Kimura, Kazumitsu Ueda, Noriyuki Kioka

2014年度日本農芸化学会関西支部大会日本農芸化学会創立90周年・関西支部創立80周年記念大会「食と生命を科学して80周年?そして未来へ」, 2014
Focal adhesion protein vinexin is involved in chondrocyte differentiation

Yuki Wantani, Yasuhisa Kimura, Kazumitsu Ueda, Noriyuki Kioka

第87回日本生化学会大会, 2014
Reconstitution of human ABCA1 in nanodiscs for the functional analysis

Takaaki Kawanobe, Yasuhisa Kimura, Noriyuki Kioka, Kaztumitsu Ueda

2014 Annual Meeting of The American Society for Cell Biology, 2014

Research Projects

ABCA1による高密度リポ蛋白質(HDL)産生メカニズムの可視化

科学研究費助成事業, 基盤研究(A), 日本学術振興会, Apr, 2023 - Mar, 2026

植田和光, 木村泰久, 難波啓一, 古寺哲幸
Functional characterization of nascent HDL generation

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2022 - Mar, 2026

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