2014年5月
The role of the interaction of the vinculin proline-rich linker region with vinexin α in sensing the stiffness of the extracellular matrix
Journal of Cell Science
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- 巻
- 127
- 号
- 9
- 開始ページ
- 1875
- 終了ページ
- 1886
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1242/jcs.133645
Although extracellular matrix (ECM) stiffness is an important aspect of the extracellular microenvironment and is known to direct the lineage specification of stem cells and affect cancer progression, the molecular mechanisms that sense ECM stiffness have not yet been elucidated. In this study, we show that the proline-rich linker (PRL) region of vinculin and the PRL-region-binding protein vinexin are involved in sensing the stiffness of ECM substrates. A rigid substrate increases the level of cytoskeleton-associated vinculin, and the fraction of vinculin stably localizing at focal adhesions (FAs) is larger on rigid ECM than on soft ECM. Mutations in the PRL region or the depletion of vinexin expression impair these responses to ECM stiffness. Furthermore, vinexin depletion impairs the stiffness-dependent regulation of cell migration. These results suggest that the interaction of the PRL region of vinculin with vinexin a plays a crucial role in sensing ECM stiffness and in mechanotransduction. © 2014. Published by The Company of Biologists Ltd.
- リンク情報
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- DOI
- https://doi.org/10.1242/jcs.133645
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24554436
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000335814800003&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899715850&origin=inward
- ID情報
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- DOI : 10.1242/jcs.133645
- ISSN : 0021-9533
- eISSN : 1477-9137
- PubMed ID : 24554436
- SCOPUS ID : 84899715850
- Web of Science ID : WOS:000335814800003