MISC

2014年5月

The role of the interaction of the vinculin proline-rich linker region with vinexin α in sensing the stiffness of the extracellular matrix

Journal of Cell Science
  • Hiroshi Yamashita
  • ,
  • Takafumi Ichikawa
  • ,
  • Daisuke Matsuyama
  • ,
  • Yasuhisa Kimura
  • ,
  • Kazumitsu Ueda
  • ,
  • Kazumitsu Ueda
  • ,
  • Susan W. Craig
  • ,
  • Ichiro Harada
  • ,
  • Noriyuki Kioka

127
9
開始ページ
1875
終了ページ
1886
記述言語
英語
掲載種別
DOI
10.1242/jcs.133645

Although extracellular matrix (ECM) stiffness is an important aspect of the extracellular microenvironment and is known to direct the lineage specification of stem cells and affect cancer progression, the molecular mechanisms that sense ECM stiffness have not yet been elucidated. In this study, we show that the proline-rich linker (PRL) region of vinculin and the PRL-region-binding protein vinexin are involved in sensing the stiffness of ECM substrates. A rigid substrate increases the level of cytoskeleton-associated vinculin, and the fraction of vinculin stably localizing at focal adhesions (FAs) is larger on rigid ECM than on soft ECM. Mutations in the PRL region or the depletion of vinexin expression impair these responses to ECM stiffness. Furthermore, vinexin depletion impairs the stiffness-dependent regulation of cell migration. These results suggest that the interaction of the PRL region of vinculin with vinexin a plays a crucial role in sensing ECM stiffness and in mechanotransduction. © 2014. Published by The Company of Biologists Ltd.

リンク情報
DOI
https://doi.org/10.1242/jcs.133645
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24554436
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000335814800003&DestApp=WOS_CPL
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899715850&origin=inward
ID情報
  • DOI : 10.1242/jcs.133645
  • ISSN : 0021-9533
  • eISSN : 1477-9137
  • PubMed ID : 24554436
  • SCOPUS ID : 84899715850
  • Web of Science ID : WOS:000335814800003

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