2016年6月
Efficient In Situ Regeneration of NADH Mimics by an Artificial Metalloenzyme
ACS CATALYSIS
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- 巻
- 6
- 号
- 6
- 開始ページ
- 3553
- 終了ページ
- 3557
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acscatal.6b00258
- 出版者・発行元
- AMER CHEMICAL SOC
NADH mimics (mNADHs) have been shown to accelerate and orthogonally activate ene reductase-catalyzed reactions. However, existing regeneration methods of NAD(P)H fail for mNADHs. Catalysis with artificial metalloenzymes based on streptavidin (Say) variants and a biotinylated iridium cofactor enable mNADH regeneration with formate. This regeneration can be coupled with ene reductase-catalyzed asymmetric reduction of a,fi-unsaturated compounds, because of the protective compartmentalization of the organometallic cofactor. With 10 mol % mNAD+, a preparative scale reaction (>100 mg) gave full conversion with 98% ee, where TTNs reached 2000, with respect to the Ir cofactor under ambient atmosphere in aqueous medium.
- リンク情報
- ID情報
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- DOI : 10.1021/acscatal.6b00258
- ISSN : 2155-5435
- ORCIDのPut Code : 38720916
- Web of Science ID : WOS:000377326700018