Trehalose is a disaccharide consisting of 2 glucose units linked in an alpha 1,1-glycosidic bond. Pre-exercise trehalose ingestion enhances exercise performance within 30 minutes. Enhanced performance was hypothesized to be due to a mouth rinse effect. A 3-arm double-blind crossover trial was conducted to test this hypothesis. Ten healthy male collegiate distance runners rinsed their mouths with either trehalose (6% w/v) or maltose (6% w/v) or acesulfame potassium (0.04 mg/mL) for 5 seconds and then performed an exercise assessment composed of 6-second peak power and endurance tests. Trehalose induced the highest mean power output ( P < .01) in peak power tests. In the endurance test, trehalose consistently showed higher mean power output than maltose. The 3 test drinks displayed indistinguishable sweetness and were expected to activate receptors for sweetness (T1R2-T1R3) with the same intensity. Trehalose activates taste receptors T1R1-T1R3, T1R3-T1R3 homodimer, and T1R2-T1R3, whereas sucrose activates only T1R2-T1R3. Therefore, a difference in mouth rinse effect might be due to a specific receptor in the oral cavity that recognizes differences between trehalose and maltose.