Jul, 2015
Cauliflower mosaic virus Tav protein induces leaf chlorosis in transgenic tobacco through a host response to virulence function of Tav
JOURNAL OF GENERAL PLANT PATHOLOGY
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- Volume
- 81
- Number
- 4
- First page
- 261
- Last page
- 270
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1007/s10327-015-0600-4
- Publisher
- SPRINGER JAPAN KK
To study the precise mechanisms underlying the chlorosis caused by plant viruses, we previously established a synchronous experimental system using transgenic plants expressing Cauliflower mosaic virus multifunctional protein, Tav (transactivator/viroplasmin), under the control of an artificially inducible promoter. Shortly after the induction of Tav expression, pathogenesis-related protein (PR) 1a gene expression is upregulated in the transgenic tobacco lines, which show visible chlorosis within a week. The present study showed that the expression of Tav also induces some salicylic acid (SA)- and ethylene-responsive PR genes. In contrast to transiently expressed Tav, which suppressed Agrobacterium-induced and SA-induced PR1a expression, the artificial induction of Tav from the transgene did not affect SA-induced PR1a expression, rather it alone induced PR1a expression. In a deletion analysis, chlorosis and PR1a induction function in transgenic tobacco were mapped to a region in Tav that had been shown to have a role in pathogenesis in a susceptible host, elicitation of the hypersensitive response in a resistant host, suppression of RNA silencing, and the suppression of Tomato bushy stunt virus P19-mediated cell death in tobacco. The results suggest that Tav-induced chlorosis results from a host response, which accompanies PR1a induction, to pathogenic function of Tav.
- Link information
- ID information
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- DOI : 10.1007/s10327-015-0600-4
- ISSN : 1345-2630
- eISSN : 1610-739X
- Web of Science ID : WOS:000358196700001