論文

査読有り 国際誌
2019年6月

Evaluation of IgA1 O-glycosylation in Henoch-Schönlein Purpura Nephritis Using Mass Spectrometry.

Transplantation proceedings
  • Shigeaki Nakazawa
  • Ryoichi Imamura
  • Masataka Kawamura
  • Taigo Kato
  • Toyofumi Abe
  • Hirotsugu Iwatani
  • Kazuaki Yamanaka
  • Motohide Uemura
  • Hidefumi Kishikawa
  • Kenji Nishimura
  • Michiko Tajiri
  • Yoshinao Wada
  • Norio Nonomura
  • 全て表示

51
5
開始ページ
1481
終了ページ
1487
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.transproceed.2019.01.122

BACKGROUND: Glomerular deposition of IgA1 is a common feature of Henoch-Schönlein purpura nephritis (HSPN) and is indistinguishable from that seen in IgA nephropathy (IgAN). Serum IgA1 is abnormally O-glycosylated in IgA nephropathy, which may contribute to the development of glomerular injury. Abnormal O-glycosylated IgA1 was also detected in HSPN using lectin enzyme-linked immunosorbent assay; however, this method cannot provide the exact structural information of O-glycans. Mass spectrometry is an effective means of quantification of O-glycans, and there is no report to evaluate IgA1 O-glycans in HSPN using mass spectrometry. MATERIALS AND METHODS: We investigated O-glycosylation profile in serum IgA1 from 7 HSPN recipients, 26 IgAN recipients, 25 recipients with other kidney diseases (OKDs), and 26 normal healthy donors using mass spectrometry. RESULTS: Of the 14 GalNac-Gal combinations detected using mass spectrometry, the percentage of the only 6GalNAc-2Gal combination was significantly different between HSPN and IgAN. The percentage of GalNAc 3 in HSPN recipients was significantly higher than that in OKDs recipients and healthy donors (P = .0027 and P < .0001, respectively). Inversely, the percentage of GalNAc 5 in HSPN recipients was significantly lower than that in OKDs recipients and healthy donors (P = .0008, P < .0001, respectively). Moreover, the Gal content and the Gal/GalNAc ratio of HSPN recipients were significantly lower than OKDs recipients and healthy donors. CONCLUSIONS: Examination of Henoch-Schönlein purpura recipients revealed that the number of GalNAc fell and the Gal attachment to GalNAc was reduced compared to other kidney diseases and healthy donors. The IgA1 O-glycosylation profile of HSPN was very similar to that of IgAN.

リンク情報
DOI
https://doi.org/10.1016/j.transproceed.2019.01.122
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31084922
ID情報
  • DOI : 10.1016/j.transproceed.2019.01.122
  • PubMed ID : 31084922

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