論文

2015年10月

Establishment of Sandwich Enzyme-Linked ImmunoSorbent Assay (ELISA) System for Quantitation of CD81

JOURNAL OF HARD TISSUE BIOLOGY
  • Shiho Hatanaka
  • ,
  • Hiroki Mihara
  • ,
  • Sayuki Kohno
  • ,
  • Erika Fujimoto
  • ,
  • Miyuki Tanaka
  • ,
  • Motoharu Takehara
  • ,
  • Tsutomu Yamasaki
  • ,
  • Tohru Nakanishi

24
4
開始ページ
401
終了ページ
404
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2485/jhtb.24.401
出版者・発行元
JOURNAL HARD TISSUE BIOLOGY

CD81 belongs to a family of cell-surface proteins (tetraspanins) that have four transmembrane domains and two outer-membrane loops. CD81 has been shown to be up-regulated in rheumatoid arthritis (RA) synoviocytes and is involved in the progression of RA through the promotion of synoviolin expression. Recently, we showed that small interfering RNA targeting CD81 (CD81 siRNA) ameliorates paw swelling in collagen-induced arthritic (CIA) rats. CD81 siRNA also decreases the expression of TNF-alpha in SW982 cells. Previoitsly, we established hybridoma cell lines producing monoclonal antibodies (MAbs) against CD81, which decrease the proliferation of rat C6 glioma cells. Here, we established a sandwich enzyme-linked immunosorbent assay (ELISA) system for the quantitation of CD81 using these MAbs.

リンク情報
DOI
https://doi.org/10.2485/jhtb.24.401
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000363832700013&DestApp=WOS_CPL
ID情報
  • DOI : 10.2485/jhtb.24.401
  • ISSN : 1341-7649
  • Web of Science ID : WOS:000363832700013

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