論文

査読有り 国際誌
2007年5月

Growth hormone suppresses the expression of IGFBP-5, and promotes the IGF-I-induced phosphorylation of Akt in bovine mammary epithelial cells.

Domestic animal endocrinology
  • Kazuhito Sakamoto
  • ,
  • Tomoki Yano
  • ,
  • Takuya Kobayashi
  • ,
  • Akihiko Hagino
  • ,
  • Hisashi Aso
  • ,
  • Yoshiaki Obara

32
4
開始ページ
260
終了ページ
72
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.domaniend.2006.03.005
出版者・発行元
ELSEVIER SCIENCE INC

Growth hormone (GH) plays a specific role to inhibit apoptosis in the bovine mammary gland through the insulin-like growth factor (IGF)-I system, however, the mechanism of GH action is poorly understood. In this study, we show that GH dramatically inhibits the expression of IGFBP-5, and GH along with IGF-I enhanced the phosphorylation of Akt through the reduction of IGF binding protein (IGFBP)-5. To determine how GH affects Akt through IGF-I in bovine mammary epithelial cells (BMECs), we examined the phosphorylation of Akt in GH treated BMECs and found that IGF-I induced phosphorylation of Akt was significantly enhanced by the treatment with GH. We demonstrated that GH reduces mRNA and protein expression of IGFBP-5 in BMECs, but it does not affect the expression of IGFBP-3. To determine that the enhanced effect of the Akt phosphorylation by the treatment of GH is due to the inhibition of the expression of IGFBP-5, we examined the effect of IGFBP-3 and -5 on the phosphorylation of Akt through IGF-I in the GH-treated BMECs. The phosphorylation of Akt was inhibited in a dose-dependent manner when IGFBP-5 was added at varying concentrations and was also inhibited in the presence of IGFBP-3. The results of this study suggest that GH plays an important role on mammary gland involution in bovine mammary epithelial cells.

リンク情報
DOI
https://doi.org/10.1016/j.domaniend.2006.03.005
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16698222
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000245414500002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.domaniend.2006.03.005
  • ISSN : 0739-7240
  • PubMed ID : 16698222
  • Web of Science ID : WOS:000245414500002

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