論文

査読有り 筆頭著者
2016年6月

The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Yasuhiro Morita
  • ,
  • Masayasu Taniguchi
  • ,
  • Fuminori Tanihara
  • ,
  • Aya Ito
  • ,
  • Zhao Namula
  • ,
  • Lanh Thi Kim Do
  • ,
  • Mitsuhiro Takagi
  • ,
  • Tatsuya Takemoto
  • ,
  • Takeshige Otoi

78
6
開始ページ
1019
終了ページ
1023
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.15-0658
出版者・発行元
JAPAN SOC VET SCI

The changes triggered by sperm-induced activation of oocytes, which are required for normal oocyte development, can be mediated by other agents, thereby inducing the parthenogenesis. In this study, we exposed porcine oocytes to 1 mM Ca-EDTA, a metal-ion chelator, at various intervals during 48 hr of in vitro maturation to determine the optimum period of Ca-EDTA treatment for parthenogenetic activation. When the oocytes were cultured with or without Ca-EDTA from 36 hr (post-12), 24 hr (post-24), 12 hr (post-36) and 0 hr (post-48) after the start of maturation culture, the blastocyst formation rates were significantly higher (P<0.05) in the post-24, post-36 and post-48 groups (3.3%, 4.0% and 2.6%, respectively) than those in the control group without treatment (0%). Furthermore, when the oocytes were cultured with Ca-EDTA for 0 hr (control), 12 hr (pre-12), 24 hr (pre-24), 36 hr (pre-36) and 48 hr (pre-48) from the start of maturation culture, the oocytes formed blastocysts only in the pre-36 and pre-48 groups (0.4% or 0.8%, respectively). Pronuclei (<66.7%) were observed only when the periods of Ca-EDTA treatment were more than 12 hr during maturation culture. In the control group, no pronuclei were detected. Our findings demonstrate that porcine immature oocytes can be parthenogenetically activated by Ca-EDTA treatment for at least 24 hr to 36 hr during maturation culture, leading to pronucleus formation followed by the formation of blastocysts.


リンク情報
DOI
https://doi.org/10.1292/jvms.15-0658
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26947170
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000379195600014&DestApp=WOS_CPL

エクスポート
BibTeX RIS