論文

査読有り
2015年12月

EFFECTS OF DIBUTYRYL CYCLIC ADENOSINE MONOPHOSPHATE AND HUMAN CHORIONIC GONADOTROPIN ON THE FORMATION OF ANTRAL FOLLICLE-LIKE STRUCTURES BY BOVINE CUMULUS-OOCYTE COMPLEXES

ACTA VETERINARIA HUNGARICA
  • Tetsushi Ono
  • ,
  • Asako Takaoka
  • ,
  • Yasuhiro Morita
  • ,
  • Lanh Thi Kim Do
  • ,
  • Fuminori Tanihara
  • ,
  • Masayasu Taniguchi
  • ,
  • Takeshige Otoi

63
4
開始ページ
485
終了ページ
498
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1556/004.2015.045
出版者・発行元
AKADEMIAI KIADO RT

This study evaluated the effect of dibutyryl cyclic adenosine monophosphate (dbcAMP) and human chorionic gonadotropin (hCG) on the formation of antral follicle-like structures (AFLSs) and on the meiotic status of bovine cumulus-oocyte complexes (COCs) embedded in collagen gel. Supplementation with dbcAMP increased the mean diameter of AFLSs during days 4-8 of culture compared with that of control COCs, irrespective of the concentration of dbcAMP used (0.5-2.0 mM). When the embedded COCs were cultured for 8 days with hCG, the diameters of AFLSs after 4 days of culture tended to be lower in the supplemented COCs than in the control COCs without hCG, irrespective of the concentration used (1-100 IU/mL). Supplementation with 10 IU/mL hCG increased the concentrations of anti-Mullerian hormone but not progesterone and oestradiol in the culture medium after 4 days of culture. Almost all oocytes collected from AFLSs had resumed meiosis by the end of culture, irrespective of supplementation of dbcAMP and hCG. These results indicate that although dbcAMP had a positive effect on AFLS formation and development, supplementation with hCG was detrimental. Moreover, hCG supplementation did not influence the luteinisation of granulosa cells in the AFLS for 4 days after the start of culture.


リンク情報
DOI
https://doi.org/10.1556/004.2015.045
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26599095
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000365351400008&DestApp=WOS_CPL

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