論文

査読有り
2015年6月

Astaxanthin present in the maturation medium reduces negative effects of heat shock on the developmental competence of porcine oocytes

REPRODUCTIVE BIOLOGY
  • Lanh Thi Kim Do
  • ,
  • Vien Viet Luu
  • ,
  • Yasuhiro Morita
  • ,
  • Masayasu Taniguchi
  • ,
  • Masahiro Nii
  • ,
  • Augustine T. Peter
  • ,
  • Takeshige Otoi

15
2
開始ページ
86
終了ページ
93
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.repbio.2015.01.002
出版者・発行元
INST ANIMAL REPRODUCTION FOOD RESEARCH

Astaxanthin, one of the most common carotenoids, elicits antioxidant effects on cellular viability and embryonic development. This study was conducted to investigate the effects of astaxanthin on maturation, fertilization and development of porcine oocytes matured in vitro under heat stress conditions, and then fertilized and cultured under standard conditions. Porcine oocytes were cultured in maturation medium supplemented with different concentrations of astaxanthin (0, 0.25, 0.5 or 1 ppm) for 46 h at either 38.5 or 41 degrees C. In comparison to oocytes cultured at 38.5 degrees C, the exposure of porcine oocytes to 41.0 degrees C during in vitro maturation (IVM) significantly inhibited maturation and development of fertilized oocytes to the blastocyst stage. Supplementation of maturation medium with astaxanthin (0.5 ppm) significantly improved oocyte maturation, fertilization and development to the blastocysts stage in both oocyte groups. However, the total cell number and apoptosis index of blastocysts did not differ among groups. Moreover, astaxanthin (0.5 ppm) significantly increased the rate of oocytes that reached metaphase II and decreased proportion of apoptotic oocytes exposed to H2O2 (1.0 mM) during NM. In summary, we demonstrated that supplementation of maturation medium with astaxanthin (0.5 ppm) exerted antioxidative effects and improved the ability of maturation, fertilization, and development of porcine oocytes exposed to heat stress. (C) 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Sp. z o.o. All rights reserved.

Web of Science ® 被引用回数 : 28

リンク情報
DOI
https://doi.org/10.1016/j.repbio.2015.01.002
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26051456
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000356561100003&DestApp=WOS_CPL

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