論文

査読有り
2014年6月

CELL CYCLE ANALYSIS AND INTERSPECIES NUCLEAR TRANSFER OF CAT CELLS TREATED WITH CHEMICAL INHIBITORS

ACTA VETERINARIA HUNGARICA
  • Manita Wittayarat
  • ,
  • Akira Fujiwara
  • ,
  • Kaywalee Chatdarong
  • ,
  • Mongkol Techakumphu
  • ,
  • Yoko Sato
  • ,
  • Fuminori Tanihara
  • ,
  • Yasuhiro Morita
  • ,
  • Masayasu Taniguchi
  • ,
  • Takeshige Otoi

62
2
開始ページ
233
終了ページ
242
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1556/AVet.2013.050
出版者・発行元
AKADEMIAI KIADO RT

This study investigated the effect of chemical inhibitors on the cell-cycle synchronisation in cat fibroblast cells and evaluated the development of interspecies embryos reconstructed from cat donor cells and enucleated bovine oocytes. Cat fibroblast cells were treated with 15 mu g/mL roscovitine or 0.05 mu g/mL demecolcine prior to cell cycle analysis and nuclear transfer. The percentage of cat fibroblast cells arrested at the G0/G1 phase in the roscovitine group was similar to that in the control group without any treatment. The percentage of cells arrested at the G2/M phase was significantly higher in the demecolcine group than in the control group. The fusion rate of interspecies couplets was significantly greater in the roscovitine group than in the control group. Most embryos stopped the development at the 2- or 4-cell stage, and none developed into blastocysts. Chemical inhibitor-induced donor cell cycle synchronisation did not overcome developmental arrest in interspecies cloned embryos.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1556/AVet.2013.050
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24334073
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000336048800008&DestApp=WOS_CPL

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