論文

査読有り
2013年8月

Histone Deacetylase Inhibitor Improves the Development and Acetylation Levels of Cat-Cow Interspecies Cloned Embryos

CELLULAR REPROGRAMMING
  • Manita Wittayarat
  • ,
  • Yoko Sato
  • ,
  • Lanh Thi Kim Do
  • ,
  • Yasuhiro Morita
  • ,
  • Kaywalee Chatdarong
  • ,
  • Mongkol Techakumphu
  • ,
  • Masayasu Taniguchi
  • ,
  • Takeshige Otoi

15
4
開始ページ
301
終了ページ
308
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/cell.2012.0094
出版者・発行元
MARY ANN LIEBERT, INC

Abnormal epigenetic reprogramming, such as histone acetylation, might cause low efficiency of interspecies somatic cell nuclear transfer (iSCNT). This study was conducted to evaluate the effects of trichostatin A (TSA) on the developmental competence and histone acetylation of iSCNT embryos reconstructed from cat somatic cells and bovine cytoplasm. The iSCNT cat and parthenogenetic bovine embryos were treated with various concentrations of TSA (0, 25, 50, or 100 nM) for 24 h, respectively, following fusion and activation. Treatment with 50nM TSA produced significantly higher rates of cleavage and blastocyst formation (84.3% and 4.6%, respectively) of iSCNT embryos than the rates of non-TSA-treated iSCNT embryos (63.8% and 0%, respectively). Similarly, the treatment of 50nM TSA increased the blastocyst formation rate of parthenogenetic bovine embryos. The acetylation levels of histone H3 lysine 9 (H3K9) in the iSCNT embryos with the treatment of 50nM TSA were similar to those of in vitro-fertilized embryos and significantly higher (p<0.05) than those of non-TSA-treated iSCNT embryos (control), irrespective of the embryonic development stage (two-cell, four-cell, and eight-cell stages). These results indicated that the treatment of 50nM TSA postfusion was beneficial for development to the blastocyst stage of iSCNT cat embryos and correlated with the increasing levels of acetylation at H3K9.

Web of Science ® 被引用回数 : 7

リンク情報
DOI
https://doi.org/10.1089/cell.2012.0094
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23790014
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000322324100005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1089/cell.2012.0094
  • ISSN : 2152-4971
  • PubMed ID : 23790014
  • Web of Science ID : WOS:000322324100005

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