論文

査読有り
2010年

Effect of Roscovitine Pretreatment on the Meiotic Maturation of Bovine Oocytes and their Subsequent Development after Somatic Cell Nuclear Transfer

JOURNAL OF ANIMAL AND VETERINARY ADVANCES
  • Yukine Kaedei
  • ,
  • Akira Fujiwara
  • ,
  • Aya Ito
  • ,
  • Fuminori Tanihara
  • ,
  • Yasuhiro Morita
  • ,
  • Keisuke Hanatate
  • ,
  • Vien Luu Viet
  • ,
  • Zhao Namula
  • ,
  • Takeshige Otoi

9
22
開始ページ
2848
終了ページ
2853
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
MEDWELL ONLINE

Roscovitine, a specific inhibitor of M-phase promoting factor kinase activity was used to inhibit the completion of meiotic maturation of bovine oocytes. The objectives of this study were to evaluate the nuclear maturation of bovine oocytes pre-cultured with various concentrations (0, 50, 100 and 200 mu M) of roscovitine before in vitro Maturation (IVM) and to examine the development of Somatic Cell Nuclear Transfer (SCNT) embryos derived from the oocytes pre-cultured with roscovitine. Before IVM, 72% of oocytes that were cultured without roscovitine (control) had reached the Metaphase II (MII) stage whereas culture with roscovitine decreased the rates of oocytes reaching MII (11-27%). After IVM, the maturation rate of oocytes pre-cultured with 200 mu M roscovitine was significantly higher than that of control oocytes (79 vs. 58%). Moreover, significantly more oocytes extruded the first polar body in the 50 mu M roscovitine group than in the control group (64 vs. 51%). The rate of blastocyst formation of reconstructed embryos derived from oocytes pre-cultured with 50 mu M roscovitine was significantly higher than that from the control oocytes (14 vs. 6%). In this study, the addition of roscovitine to culture medium delays the completion of meiotic maturation of bovine oocytes and the cytoplasm derived from oocytes pre-cultured under meiotic inhibition can support the development of SCNT embryos.

Web of Science ® 被引用回数 : 6

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000284231600013&DestApp=WOS_CPL
ID情報
  • ISSN : 1680-5593
  • Web of Science ID : WOS:000284231600013

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