論文

査読有り
2002年10月

Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry

NATURE
  • E Lasonder
  • Y Ishihama
  • JS Andersen
  • AMW Vermunt
  • A Pain
  • RW Sauerwein
  • WMC Eling
  • N Hall
  • AP Waters
  • HG Stunnenberg
  • M Mann
  • 全て表示

419
6906
開始ページ
537
終了ページ
542
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/nature01111
出版者・発行元
NATURE PUBLISHING GROUP

The annotated genomes of organisms define a 'blueprint' of their possible gene products. Post-genome analyses attempt to confirm and modify the annotation and impose a sense of the spatial, temporal and developmental usage of genetic information by the organism. Here we describe a large-scale, high-accuracy (average deviation less than 0.02 Da at 1,000 Da) mass spectrometric proteome analysis(1-3) of selected stages of the human malaria parasite Plasmodium falciparum. The analysis revealed 1,289 proteins of which 714 proteins were identified in asexual blood stages, 931 in gametocytes and 645 in gametes. The last two groups provide insights into the biology of the sexual stages of the parasite, and include conserved, stage-specific, secreted and membrane-associated proteins. A subset of these proteins contain domains that indicate a role in cell-cell interactions, and therefore can be evaluated as potential components of a malaria vaccine formulation. We also report a set of peptides with significant matches in the parasite genome but not in the protein set predicted by computational methods.

リンク情報
DOI
https://doi.org/10.1038/nature01111
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12368870
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000178348400051&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/nature01111
  • ISSN : 0028-0836
  • PubMed ID : 12368870
  • Web of Science ID : WOS:000178348400051

エクスポート
BibTeX RIS