2019年10月
A new in vitro muscle contraction model and its application for analysis of mTORC1 signaling in combination with contraction and beta-hydroxy-beta-methylbutyrate administration.
Bioscience, biotechnology, and biochemistry
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- 巻
- 83
- 号
- 10
- 開始ページ
- 1851
- 終了ページ
- 1857
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2019.1625261
Several food constituents augment exercise-induced muscle strength improvement; however, the detailed mechanism underlying these combined effects is unknown because of the lack of a cultured cell model for evaluating the contraction-induced muscle protein synthesis level. Here, we aimed to establish a new in vitro muscle contraction model for analyzing the activation of mammalian target of rapamycin complex 1 (mTORC1) signaling. We adopted the tetanic electric stimulation of 50 V at 100 Hz for 10 min in L6.C11 myotubes. Akt, ERK1/2, and p70S6K phosphorylation increased significantly after electrical pulse stimulation (EPS), compared to untreated cells. Next, we used this model to analyze mTORC1 signaling in combination with exercise and beta-hydroxy-beta-methylbutyrate (HMB), an l-leucine metabolite. p70S6K phosphorylation increased significantly in the EPS+HMB group compared to that in the EPS-alone group. These findings show that our model could be used to analyze mTORC1 signaling and that HMB enhances muscle contraction-activated mTORC1 signaling.
- リンク情報
- ID情報
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- DOI : 10.1080/09168451.2019.1625261
- ISSN : 0916-8451
- PubMed ID : 31159662