Pseudo random sequences play an important role in cryptography and network security system. This paper proposes a new approach for generation of pseudo random sequence over odd characteristic field. The sequence is generated by applying a primitive polynomial over odd characteristic field, trace function and mobius function. Then, some important properties of the newly generated sequence such as period, autocorrelation and cross-correlation have been studied in this work. The properties of the generated sequence are evaluated on various bit length of odd characteristics. Finally, the experimental results are compared with existing works which show the superiority of the proposed sequence over existing ones.