MISC

2019年5月1日

"Smart Eye Camera": An innovative technique to evaluate tear film breakup time in a murine dry eye disease model

PLoS ONE
  • Eisuke Shimizu
  • ,
  • Yoko Ogawa
  • ,
  • Hiroyuki Yazu
  • ,
  • Naohiko Aketa
  • ,
  • Fan Yang
  • ,
  • Mio Yamane
  • ,
  • Yasunori Sato
  • ,
  • Yutaka Kawakami
  • ,
  • Kazuo Tsubota

14
DOI
10.1371/journal.pone.021513

© 2019 Shimizu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and is capable of evaluating TFBUT in a murine DED model. We compared images taken by existing devices and the Smart Eye Camera in a graft-versus-host disease-related DED murine model. We observed that the quality of the images obtained by the Smart Eye Camera were sufficient for practical use. Moreover, this new technique could be used to obtain measurements for several consecutive ocular phenotypes in a variety of environments. Here, we demonstrate the effectiveness of our new invention in the examination of ocular phenotypes, including TFBUT in a murine model. We highlight the potential for future translational studies adopting the Smart Eye Camera in clinical settings.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.021513
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31071120
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-85065654187&origin=inward
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065654187&origin=inward