Yuko Fukata

Last updated: Nov 20, 2019 at 13:11

Name	Yuko Fukata
E-mail	yfukatanips.ac.jp
Affiliation	National Institutes of Natural Sciences National Institute for Physiological Sciences
Research funding number	40416186
ORCID ID	0000-0001-7724-8643

Research Interests

LGI1(1)

Research Areas

Neuroscience / Neuroscience in general /
Neuroscience / Neurochemistry/Neuropharmacology /

Academic & Professional Experience

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Dec 2007

Today

Associate Professor, Division of Membrane Physiology, National Institute for Physiological Sciences (NIPS), National Institutes of Natural Sciences (NINS)

Apr 2006

May 2007

Laboratory of Genomics and Proteomics, National Center for Geriatrics and Gerontology (NCGG), Research Institute

Apr 2000

Mar 2003

JSPS (PD), Department of Cell Pharmacology, Nagoya University Graduate School of Medicine

Apr 1997

Mar 2000

JSPS (DC1), NAIST

Awards & Honors

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2007

Career Development Awards, Human Frontier Science Program Organization

Published Papers

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Dynamic palmitoylation determines microdomain localization of two DKK1 receptors, CKAP4 and LRP6, and regulates DKK1 signaling.

Sada R, Kimura H, Fukata Y, Fukata M, Yamamoto H, Kikuchi A

Sci Signaling 12 eaat9519 Nov 2019 [Refereed]

ABHD10 is an S-depalmitoylase affecting redox homeostasis through peroxiredoxin-5.

Cao Y, Qiu T, Kathayat R, Azizi SA, Fukata Y, Fukata M, Rice P, Dickinson BC

Nat Chem Biol 15 1232-1240 Nov 2019 [Refereed]

Targeting CCR5 trafficking to inhibit HIV-1 infection.

Boncompain G, Herit F, Tessier S, Lescure A, Del Nery E, Gestraud P, Staropoli I, Fukata Y, Fukata M, Brelot A, Niedergang F, Perez F

Science Advances 5 eaax0821 Nov 2019 [Refereed]

Deleted in colorectal cancer (netrin-1 receptor) antibodies and limbic encephalitis in a cat with hippocampal necrosis.

Hasegawa D, Ohnishi Y, Koyama E, Matsunaga S, Ohtani S, Nakanishi A, Shiga T, Chambers JK, Uchida K, Yokoi N, Fukata Y, Fukata M

J Vet Intern Med 33 1440-1445 May 2019 [Refereed]

Systematic Screening of Depalmitoylating Enzymes and Evaluation of Their Activities by the Acyl-PEGyl Exchange Gel-Shift (APEGS) Assay.

Kanadome T, Yokoi N, Fukata Y, Fukata M

Methods Mol Biol 2009 83-98 2019 [Refereed]

Branched sialylated N-glycans are accumulated in brain synaptosomes and interact with Siglec-H.

Handa-Narumi M, Yoshimura T, Konishi H, Fukata Y, Manabe Y, Tanaka K, Bao GM, Kiyama H, Fukase K, Ikenaka K

Cell Struct Funct Jul 2018 [Refereed]

Long-term clinical follow-up of a patient with non-paraneoplastic cerebellar ataxia associated with anti-mGluR1 autoantibodies.

Yoshikura N, Kimura A, Fukata M, Fukata Y, Yokoi N, Harada N, Hayashi Y, Inuzuka T, Shimohata T

J Neuroimmunol. 319 63-67 Jun 2018 [Refereed]

Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22.

Yamagata A, Miyazaki Y, Yokoi N, Shigematsu H, Sato Y, Goto-Ito S, Maeda A, Goto T, Sanbo M, Hirabayashi M, Shirouzu M, Fukata Y, Fukata M, Fukai S

Nat Commun 9(1) 1546 Apr 2018 [Refereed]

Neurobiology of autoimmune encephalitis.

Fukata M, Yokoi N, Fukata Y

Curr Opin Neurobiol 48 1-8 Feb 2018 [Refereed]

In situ screening for postsynaptic cell adhesion molecules during synapse formation.

Uemura T, Shiroshima T, Maeda A, Yasumura M, Shimada T, Fukata Y, Fukata M, Yoshida T

J Biochem 162(4) 295-302 Oct 2017 [Refereed]

Ivermectin activates GIRK channels in a PIP₂ -dependent, G_βγ -independent manner and an amino acid residue at the slide helix governs the activation.

Chen IS, Tateyama M, Fukata Y, Uesugi M, Kubo Y

J Physiol 595(17) 5895-5912 Sep 2017 [Refereed]

Epilepsy and synaptic proteins.

Fukata Y, Fukata M

Curr Opin Neurobiol 45 1-8 Aug 2017 [Refereed]

The LGI1-ADAM22 protein complex in synaptic transmission and synaptic disorders.

Fukata Y, Yokoi N, Miyazaki Y, Fukata M

Neurosci Res 116 39-45 Mar 2017 [Refereed]

Coupling of a voltage-gated Ca²⁺ channel homologue with a plasma membrane H⁺ -ATPase in yeast.

Cho T, Ishii-Kato A, Fukata Y, Nakayama Y, Iida K, Fukata M, Iida H

Genes Cells 22(1) 94-104 Jan 2017 [Refereed]

Identification of PSD-95 Depalmitoylating Enzymes.

Yokoi N*, Fukata Y*, Sekiya A, Murakami T, Kobayashi K, Fukata M

J Neurosci 36(24) 6431-6444 Jun 2016 [Refereed]

Dysfunctional ADAM22 implicated in progressive encephalopathy with cortical atrophy and epilepsy.

Muona M, Fukata Y, Anttonen AK, Laari A, Palotie A, Pihko H, Lönnqvist T, Valanne L, Somer M, Fukata M, Lehesjoki AE

Neurol Genet 2(1) e46 Feb 2016 [Refereed]

Local Palmitoylation Cycles and Specialized Membrane Domain Organization

Fukata Y, Murakami T, Yokoi N, Fukata M

Curr Top Membr 77 97-141 Jan 2016

The LGI1-ADAM22 protein complex directs synapse maturation through regulation of PSD-95 function

Lovero KL, Fukata Y, Granger AJ, Fukata M, Nicoll RA

Proc Natl Acad Sci U S A. 112 E4129-E4137 Jul 2015

Postsynaptic nanodomains generated by local palmitoylation cycles

Fukata M, Sekiya A, Murakami T, Yokoi N, Fukata Y

Biochem Soc Trans 43 199-204 Apr 2015

Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy

Yokoi N, Fukata Y#, Kase D, Miyazaki T, Jaegle M, Ohkawa T, Takahashi N, Iwanari H, Mochizuki Y, Hamakubo T, Imoto K, Meijer D, Watanabe M, Fukata M#

Nat Med 21 19-26 Jan 2015

Non-microtubular localizations of Microtubule-Associated Protein 6 (MAP6)

Gory-Fauré S, Windscheid V, Brocard J, Montessuit S, Tsutsumi R, Denarier E, Fukata Y, Bosc C, Delaroche J, Collomb N, Fukata M, Martinou JC, Pernet-Gallay K, Andrieux A

PLoS ONE 9 Dec 2014

Identification and characterization of GABA<inf>A</inf> receptor autoantibodies in autoimmune encephalitis

Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, Takashima H, Watanabe O, Fukata Y#, Fukata M#

J Neurosci 34 8151-8163 Jan 2014

Functional phylogenetic analysis of LGI proteins identifies an interaction motif crucial for myelination

Kegel L, Jaegle M, Driegen S, Aunin E, Leslie K, Fukata Y, Watanabe M, Fukata M, Meijer D

Development 127 1749-1756 Jan 2014

Autoantibodies to epilepsy-related LGI1 in limbic encephalitis neutralize LGI1-ADAM22 interaction and reduce synaptic AMPA receptors

Ohkawa T, Fukata Y, Yamasaki M, Miyazaki T, Yokoi N, Takashima H, Watanabe M, Watanabe O, Fukata M

J Neurosci 33 18161-18174 Nov 2013

Neuronal major histocompatibility complex class I molecules are implicated in the generation of asymmetries in hippocampal circuitry

Kawahara A, Kurauchi S, Fukata Y, Martínez-Hernández J, Yagihashi T, Itadani Y, Sho R, Kajiyama T, Shinzato N, Narusuye K, Fukata M, Luján R, Shigemoto R, Ito I

J Physiol 591 4777-4791 Oct 2013

In Silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes

Oku S, Takahashi N, Fukata Y, Fukata M

J Biol Chem 288 19816-19826 Jul 2013

Local palmitoylation cycles define activity-regulated postsynaptic subdomains

Fukata Y, Dimitrov A, Boncompain G, Vielemeyer O, Perez F, Fukata M

J Cell Biol 202 145-161 Jul 2013

Leucine-rich glioma inactivated 1 (Lgi1), an epilepsy-related secreted protein, has a nuclear localization signal and localizes to both the cytoplasm and the nucleus of the caudal ganglionic eminence neurons

Kusuzawa S, Honda T, Fukata Y, Fukata M, Kanatani S, Tanaka DH, Nakajima K

Eur J Neurosci 36 2284-2292 Aug 2012

Phosphatidylinositol 4-kinase IIα is palmitoylated by golgi-localized palmitoyltransferases in cholesterol-dependent manner

Lu D, Sun HQ, Wang H, Barylko B, Fukata Y, Fukata M, Albanesi JP, Yin HL

J Biol Chem 287 21856-21865 Jun 2012

Synaptic Plasticity Regulated by Protein-Protein Interactions and Posttranslational Modifications

Yokoi N, Fukata M, Fukata Y

Int Rev Cell Mol Biol 297 1-43 May 2012

Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases.

Levy AD, Devignot V, Fukata Y, Fukata M, Sobel A, Chauvin S

Mol Biol Cell 22(11) 1930-1942 Jun 2011 [Refereed]

Lgi2 truncation causes a remitting focal epilepsy in dogs

Seppälä EH, Jokinen TS, Fukata M, Fukata Y, Webster MT, Karlsson EK, Kilpinen SK, Steffen F, Dietschi E, Leeb T, Eklund R, Zhao X, Rilstone JJ, Lindblad-Toh K, Minassian BA, Lohi H

PLoS Genet 7 Jan 2011

Protein palmitoylation in neuronal development and synaptic plasticity.

Fukata Y, Fukata M

Nat Rev Neurosci 11(3) 161-175 Mar 2010 [Refereed]

Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy.

Fukata Y, Lovero KL, Iwanaga T, Watanabe A, Yokoi N, Tabuchi K, Shigemoto R, Nicoll RA, Fukata M

Proc Natl Acad Sci U S A 107(8) 3799-3804 Feb 2010 [Refereed]

Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity.

Shmueli A, Segal M, Sapir T, Tsutsumi R, Noritake J, Bar A, Sapoznik S, Fukata Y, Orr I, Fukata M, Reiner O

EMBO J 29(1) 107-119 Jan 2010 [Refereed]

Palmitoylation regulates epidermal homeostasis and hair follicle differentiation.

Mill P, Lee AW, Fukata Y, Tsutsumi R, Fukata M, Keighren M, Porter RM, McKie L, Smyth I, Jackson IJ

PLoS Genet 5(11) e1000748 Nov 2009 [Refereed]

Differential activity-dependent secretion of brain-derived neurotrophic factor from axon and dendrite.

Matsuda N, Lu H, Fukata Y, Noritake J, Gao H, Mukherjee S, Nemoto T, Fukata M, Poo MM

J Neurosci 29(45) 14185-14198 Nov 2009 [Refereed]

Mobile DHHC palmitoylating enzyme mediates activity-sensitive synaptic targeting of PSD-95.

Noritake J, Fukata Y, Iwanaga T, Hosomi N, Tsutsumi R, Matsuda N, Tani H, Iwanari H, Mochizuki Y, Kodama T, Matsuura Y, Bredt DS, Hamakubo T, Fukata M

J Cell Biol 186(1) 147-160 Jul 2009 [Refereed]

Dynamic protein palmitoylation in cellular signaling.

Iwanaga T, Tsutsumi R, Noritake J, Fukata Y, Fukata M

Prog Lipid Res 48(3-4) 117-127 May 2009 [Refereed]

The hydrophobic cysteine-rich domain of SNAP25 couples with downstream residues to mediate membrane interactions and recognition by DHHC palmitoyl transferases.

Greaves J, Prescott GR, Fukata Y, Fukata M, Salaun C, Chamberlain LH

Mol Biol Cell 20(6) 1845-1854 Mar 2009 [Refereed]

Identification of G protein alpha subunit-palmitoylating enzyme.

Tsutsumi R, Fukata Y, Noritake J, Iwanaga T, Perez F, Fukata M

Mol Cell Biol 29(2) 435-447 Jan 2009 [Refereed]

Fibroblast growth factor-regulated palmitoylation of the neural cell adhesion molecule determines neuronal morphogenesis.

Ponimaskin E, Dityateva G, Ruonala MO, Fukata M, Fukata Y, Kobe F, Wouters FS, Delling M, Bredt DS, Schachner M, Dityatev A

J Neurosci 28(36) 8897-8907 Sep 2008 [Refereed]

Palmitoylation and membrane interactions of the neuroprotective chaperone cysteine-string protein.

Greaves J, Salaun C, Fukata Y, Fukata M, Chamberlain LH

J Biol Chem 283(36) 25014-25026 Sep 2008 [Refereed]

Discovery of protein-palmitoylating enzymes.

Tsutsumi R, Fukata Y, Fukata M

Pflugers Arch 456(6) 1199-1206 Sep 2008 [Refereed]

GM1-ganglioside-induced Abeta assembly on synaptic membranes of cultured neurons.

Yamamoto N, Fukata Y, Fukata M, Yanagisawa K

Biochim Biophys Acta 1768(5) 1128-1137 May 2007 [Refereed]

Stargazin interacts functionally with the AMPA receptor glutamate-binding module.

Tomita S, Shenoy A, Fukata Y, Nicoll RA, Bredt DS

Neuropharmacology 52(1) 87-91 Jan 2007 [Refereed]

GODZ-mediated palmitoylation of GABA(A) receptors is required for normal assembly and function of GABAergic inhibitory synapses.

Fang C, Deng L, Keller CA, Fukata M, Fukata Y, Chen G, Lüscher B

J Neurosci 26(49) 12758-12768 Dec 2006 [Refereed]

Systematic screening for palmitoyl transferase activity of the DHHC protein family in mammalian cells.

Fukata Y, Iwanaga T, Fukata M

Methods 40(2) 177-182 Oct 2006 [Refereed]

Fukata Y, Adesnik H, Iwanaga T, Bredt DS, Nicoll RA, Fukata M

Science 313(5794) 1792-1795 Sep 2006 [Refereed]

Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase.

Fernández-Hernando C, Fukata M, Bernatchez PN, Fukata Y, Lin MI, Bredt DS, Sessa WC

J Cell Biol 174(3) 369-377 Jul 2006 [Refereed]

Protein palmitoylation by DHHC protein family

Fukata Y, Bredt DS, Fukata M

The dynamic synapse: molecular methods in ionotropic receptor biology 81-87 2006 [Refereed][Invited]

Phosphorylation by Rho kinase regulates CRMP-2 activity in growth cones.

Arimura N, Ménager C, Kawano Y, Yoshimura T, Kawabata S, Hattori A, Fukata Y, Amano M, Goshima Y, Inagaki M, Morone N, Usukura J, Kaibuchi K

Mol Cell Biol 25(22) 9973-9984 Nov 2005 [Refereed]

Tubulin and CRMP-2 complex is transported via Kinesin-1

Kimura T, Arimura N, Fukata Y, Watanabe H, Iwamatsu A, Kaibuchi K

Journal of Neurochemistry 93 1371-1382 Jun 2005

Molecular constituents of neuronal AMPA receptors.

Fukata Y, Tzingounis AV, Trinidad JC, Fukata M, Burlingame AL, Nicoll RA, Bredt DS

J Cell Biol 169(3) 399-404 May 2005 [Refereed]

Identification of PSD-95 palmitoylating enzymes.

Fukata M, Fukata Y, Adesnik H, Nicoll RA, Bredt DS

Neuron 44(6) 987-996 Dec 2004 [Refereed]

Role of the PAR-3-KIF3 complex in the establishment of neuronal polarity.

Nishimura T, Kato K, Yamaguchi T, Fukata Y, Ohno S, Kaibuchi K

Nat Cell Biol 6(4) 328-334 Apr 2004 [Refereed]

PIP3 is involved in neuronal polarization and axon formation.

Ménager C, Arimura N, Fukata Y, Kaibuchi K

J Neurochem 89(1) 109-118 Apr 2004 [Refereed]

Positive role of IQGAP1, an effector of Rac1, in actin-meshwork formation at sites of cell-cell contact.

Noritake J, Fukata M, Sato K, Nakagawa M, Watanabe T, Izumi N, Wang S, Fukata Y, Kaibuchi K

Mol Biol Cell 15(3) 1065-1076 Mar 2004 [Refereed]

Role of CRMP-2 in neuronal polarity.

Arimura N, Menager C, Fukata Y, Kaibuchi K

J Neurobiol 58(1) 34-47 Jan 2004 [Refereed]

Identification of Tau and MAP2 as novel substrates of Rho-kinase and myosin phosphatase.

Amano M, Kaneko T, Maeda A, Nakayama M, Ito M, Yamauchi T, Goto H, Fukata Y, Oshiro N, Shinohara A, Iwamatsu A, Kaibuchi K

J Neurochem 87(3) 780-790 Nov 2003 [Refereed]

CRMP-2 regulates polarized Numb-mediated endocytosis for axon growth.

Nishimura T, Fukata Y, Kato K, Yamaguchi T, Matsuura Y, Kamiguchi H, Kaibuchi K

Nat Cell Biol 5(9) 819-826 Sep 2003 [Refereed]

Translocation of Na(+),K(+)-ATPase is induced by Rho small GTPase in renal epithelial cells.

Maeda A, Amano M, Fukata Y, Kaibuchi K

Biochem Biophys Res Commun 297(5) 1231-1237 Oct 2002 [Refereed]

Axon specification in hippocampal neurons.

Fukata Y, Kimura T, Kaibuchi K

Neuroscience research 43(4) 305-315 Aug 2002 [Refereed]

CRMP-2 binds to tubulin heterodimers to promote microtubule assembly.

Fukata Y, Itoh TJ, Kimura T, Ménager C, Nishimura T, Shiromizu T, Watanabe H, Inagaki N, Iwamatsu A, Hotani H, Kaibuchi K

Nat Cell Biol 4(8) 583-591 Aug 2002 [Refereed]

Rho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells

Fukata Y, Amano M, Kaibuchi K

Trends Pharmacol Sci 22 32-39 Jan 2001

Elongation factor-1α is a novel substrate of Rho-associated kinase

Izawa T, Fukata Y, Kimura T, Iwamatsu A, Dohi K, Kaibuchi K

Biochem Biophys Res Commun 278 72-78 Nov 2000

Regulation and functions of Rho-associated kinase

Amano M, Fukata Y, Kaibuchi K

Exp Cell Res 261 44-51 Nov 2000

Phosphorylation of ERM proteins at filopodia induced by Cdc42

Nakamura N, Oshiro N, Fukata Y, Amano M, Fukata M, Kuroda S, Matsuura Y, Leung T, Lim L, Kaibuchi K

Genes to Cells 5 571-581 Aug 2000

Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility

Fukata Y, Oshiro N, Kinoshita N, Kawano Y, Matsuoka Y, Bennett V, Matsuura Y, Kaibuchi K

J Cell Biol 145 347-361 Jun 2000

Inhibition of myosin phosphatase by upregulated Rho-kinase plays a key role for coronary artery spasm in a porcine model with interleukin-1β

Kandabashi T, Shimokawa H, Miyata K, Kunihiro I, Kawano Y, Fukata Y, Higo T, Egashira K, Takahashi S, Kaibuchi K, Takeshita A

Circulation 101 1319-1323 Mar 2000

Purification and in vitro activity of Rho-associated kinase

Amano M, Fukata Y, Shimokawa H, Kaibuchi K

Methods Enzymol 325 149-155 Jan 2000

Activation of moesin and adducin by Rho-kinase downstream of Rho

Fukata Y, Oshiro N, Kaibuchi K

Biophys Chem 82 139-147 Dec 1999

Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo

Kawano Y, Fukata Y, Oshiro N, Amano M, Nakamura T, Ito M, Matsumura F, Inagaki M, Kaibuchi K

J Cell Biol 147 1023-1037 Nov 1999

Phosphorylation of moesin by Rho-associated kinase (Rho-kinase) plays a crucial role in the formation of microvilli-like structures

Oshiro N, Fukata Y, Kaibuchi K

J Biol Chem 273 34663-34666 Dec 1998

Myosin II activation promotes neurite retraction during the action of Rho and Rho-kinase

Amano M, Chihara K, Nakamura N, Fukata Y, Yano T, Shibata M, Ikebe M, Kaibuchi K

Genes to Cells 3 177-188 Aug 1998

Regulation of cytoskeleton and cell adhesions by the small GTPase Rho and its targets

Amano M, Fukata Y, Kaibuchi K

Trends Cardiovasc Med 8 162-168 May 1998

Regulation of the association of adducin with actin filaments by Rho- associated kinase (Rho-kinase) and myosin phosphatase

Kimura K, Fukata Y, Matsuoka Y, Bennett V, Matsuura Y, Okawa K, Iwamatsu A, Kaibuchi K

J Biol Chem 273 5542-5548 Mar 1998

Association of the myosin-binding subunit of myosin phosphatase and moesin: dual regulation of moesin phosphorylation by Rho-associated kinase and myosin phosphatase

Y Fukata, K Kimura, N Oshiro, H Saya, Y Matsuura, K Kaibuchi

J Cell Biol 141(2) 409-418 1998 [Refereed]

Formation of actin stress fibers and focal adhesions enhanced by Rho- kinase

Amano M, Chihara K, Kimura K, Fukata Y, Nakamura N, Matsuura Y, Kaibuchi K

Science 275 1308-1311 Feb 1997

Phosphorylation and activation of myosin by Rho-associated kinase (Rho- kinase)

Amano M, Ito M, Kimura K, Fukata Y, Chihara K, Nakano T, Matsuura Y, Kaibuchi K

J Biol Chem 271 20246-20249 Sep 1996

Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho- kinase)

Kimura K, Ito M, Amano M, Chihara K, Fukata Y, Nakafuku M, Yamamori B, Feng J, Nakano T, Okawa K, Iwamatsu A, Kaibuchi K

Science 273 245-248 Jul 1996

Association Memberships

THE JAPAN NEUROSCIENCE SOCIETY(1205) , Society for Neuroscience(1041) , The American Society for Cell Biology(100) , THE PHYSIOLOGICAL SOCIETY OF JAPAN(457)