論文

査読有り
2019年4月

Overexpression of Prunus DAM6 inhibits growth, represses bud break competency of dormant buds and delays bud outgrowth in apple plants

PLOS ONE
  • Yamane, Hisayo
  • ,
  • Wada, Masato
  • ,
  • Honda, Chikako
  • ,
  • Matsuura, Takakazu
  • ,
  • Ikeda, Yoko
  • ,
  • Hirayama, Takashi
  • ,
  • Osako, Yutaro
  • ,
  • Gao-Takai, Mei
  • ,
  • Kojima, Mikiko
  • ,
  • Sakakibara, Hitoshi
  • ,
  • Tao, Ryutaro

14
4
開始ページ
e0214788
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0214788
出版者・発行元
PUBLIC LIBRARY SCIENCE

Most deciduous fruit trees cultivated in the temperate zone require a genotype-dependent amounts of chilling exposure for dormancy release and bud break. In Japanese apricot (Prunus mume), DORMANCY-ASSOCIATED MADS-box 6 (PmDAM6) may influence chilling-mediated dormancy release and bud break. In this study, we attempted to elucidate the biological functions of PmDAM6 related to dormancy regulation by analyzing PmDAM6-overexpressing transgenic apple (Malusspp.). We generated 35S:PmDAM6 lines and chemically inducible overexpression lines, 35S:PmDAM6-GR. In both overexpression lines, shoot growth was inhibited and early bud set was observed. In addition, PmDAM6 expression repressed bud break competency during dormancy and delayed bud break. Moreover, PmDAM6 expression increased abscisic acid levels and decreased cytokinins contents during the late dormancy and bud break stages in both 35S:PmDAM6 and 35S:PmDAM6-GR. Our analysis also suggested that abscisic acid levels increased during dormancy but subsequently decreased during dormancy release whereas cytokinins contents increased during the bud break stage in dormant Japanese apricot buds. We previously revealed that PmDAM6 expression is continuously down-regulated during dormancy release toward bud break in Japanese apricot. The PmDAM6 expression pattern was concurrent with a decrease and increase in the abscisic acid and cytokinins contents, respectively, in dormant Japanese apricot buds. Therefore, we hypothesize that PmDAM6 represses the bud break competency during dormancy and bud break stages in Japanese apricot by modulating abscisic acid and cytokinins accumulation in dormant buds.

Web of Science ® 被引用回数 : 12

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0214788
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30964897
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000463876300029&DestApp=WOS_CPL

エクスポート
BibTeX RIS