論文

査読有り
2014年12月1日

Persistence of recipient-derived as well as donor-derived clones of cytomegalovirus pp65-specific cytotoxic T cells long after allogeneic hematopoietic stem cell transplantation

Transplant Infectious Disease
  • K. Terasako-Saito
  • H. Nakasone
  • Y. Tanaka
  • R. Yamazaki
  • M. Sato
  • K. Sakamoto
  • Y. Ishihara
  • K. Kawamura
  • Y. Akahoshi
  • J. Hayakawa
  • H. Wada
  • N. Harada
  • H. Nakano
  • K. Kameda
  • T. Ugai
  • R. Yamasaki
  • M. Ashizawa
  • S. I. Kimura
  • M. Kikuchi
  • A. Tanihara
  • J. Kanda
  • S. Kako
  • J. Nishida
  • Y. Kanda
  • 全て表示

16
6
開始ページ
930
終了ページ
940
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/tid.12318
出版者・発行元
Blackwell Publishing Inc.

Background: Cytomegalovirus (CMV)-specific CD8+ cytotoxic T lymphocytes (CMV-CTLs) play a crucial role in preventing CMV disease. However, the actual in vivo dynamics of CMV-CTL clones after allogeneic hematopoietic stem cell transplantation (alloHCT) are still unclear. Methods: Using a single-cell T-cell receptor repertoire analysis, we monitored clones and chimerism of CMV-CTLs in 3 CMV-seropositive alloHCT recipients from CMV-seronegative donors, with or without CMV reactivation. Results: Nearly all of the CMV-CTLs during follow-up were CD45RA-CCR7- effector memory/CD45RA+CCR7- effector T cells, and were highly matured. In each case, the use of BV gene families was restricted, especially in BV5, 7, 28, and 29. Although no common predominant CMV-CTL clones were found, several shared motifs of complementarity-determining region-3 were identified among the 3 cases
QGA in all, TGE and TDT in Case 1 and Case 2, and RDRG in Case 2 and Case 3. In all cases, CMV-CTL clones that were detected for the first time after alloHCT persisted as the dominant clones. In Case 1, without CMV reactivation, recipient-derived CMV-CTLs exclusively persisted as a dominant clone, while all CMV-CTLs in the other 2 cases, with CMV reactivation, were donor derived. Conclusion: Clone monitoring and chimerism analyses should help to further clarify novel aspects of immuno-reconstitution after alloHCT.

リンク情報
DOI
https://doi.org/10.1111/tid.12318
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25430567
ID情報
  • DOI : 10.1111/tid.12318
  • ISSN : 1399-3062
  • ISSN : 1398-2273
  • PubMed ID : 25430567
  • SCOPUS ID : 84920261134

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