論文

査読有り
2017年9月

Membrane-localized ubiquitin ligase ATL15 functions in sugar-responsive growth regulation in Arabidopsis

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Shoki Aoyama
  • ,
  • Saki Terada
  • ,
  • Miho Sanagi
  • ,
  • Yoko Hasegawa
  • ,
  • Yu Lu
  • ,
  • Yoshie Morita
  • ,
  • Yukako Chiba
  • ,
  • Takeo Sato
  • ,
  • Junji Yamaguchi

491
1
開始ページ
33
終了ページ
39
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2017.07.028
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Ubiquitin ligases play important roles in regulating various cellular processes by modulating the protein function of specific ubiquitination targets. The Arabidopsis Toxicos en Levadura (ATL) family is a group of plant-specific RING-type ubiquitin ligases that localize to membranes via their N-terminal transmembrane-like domains. To date, 91 ATL isoforms have been identified in the Arabidopsis genome, with several ATLs reported to be involved in regulating plant responses to environmental stresses. However, the functions of most ATLs remain unknown. This study, involving transcriptome database analysis, identifies ATL15 as a sugar responsive ATL gene in Arabidopsis. ATL15 expression was rapidly down-regulated in the presence of sugar. The ATL15 protein showed ubiquitin ligase activity in vitro and localized to plasma membrane and endomembrane compartments. Further genetic analyses demonstrated that the atI15 knockout mutants are insensitive to high glucose concentrations, whereas ATL15 overexpression depresses plant growth. In addition, endogenous glucose and starch amounts were reciprocally affected in the at115 knockout mutants and the ATL15 overexpressors. These results suggest that ATL15 protein plays a significant role as a membrane-localized ubiquitin ligase that regulates sugar responsive plant growth in Arabidopsis. (C) 2017 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2017.07.028
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28690153
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000408518700006&DestApp=WOS_CPL