論文

査読有り
2014年4月

Growth assay of individual pollen tubes arrayed by microchannel device

Microelectronic Engineering
  • Mitsuhiro Horade
  • ,
  • Naoki Yanagisawa
  • ,
  • Yoko Mizuta
  • ,
  • Tetsuya Higashiyama
  • ,
  • Hideyuki Arata

118
開始ページ
25
終了ページ
28
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.mee.2014.01.017
出版者・発行元
ELSEVIER SCIENCE BV

To restrict pollen tube growth to a single focal plane is an important subject to enable their accurate growth analysis under microscopic observation. In the conventional method to assay pollen tube growth, the pollen tubes grow in a disorderly manner on solid medium, rendering it impossible to observe their growth in detail. Here, we present a new method to assay pollen tube growth using poly-dimethylsiloxane microchannel device to isolate individual pollen tubes. The growth of the pollen tube is confined to the microchannel and to the same focal plane, allowing accurate microscopic observations. This methodology has the potential for analyses of pollen tube growth in microfluidic environments in response to chemical products and signaling molecules, which paves the way for various experiments on plant reproduction. (C) 2014 The Authors. Published by Elsevier B.V.

リンク情報
DOI
https://doi.org/10.1016/j.mee.2014.01.017
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201402275565404165
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000335431000006&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84894448889&origin=inward
ID情報
  • DOI : 10.1016/j.mee.2014.01.017
  • ISSN : 0167-9317
  • eISSN : 1521-3757
  • J-Global ID : 201402275565404165
  • SCOPUS ID : 84894448889
  • Web of Science ID : WOS:000335431000006

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