2022年12月21日
Target pollen isolation using automated infrared laser-mediated cell disruption
Quantitative Plant Biology
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- 巻
- 3
- 号
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1017/qpb.2022.24
- 出版者・発行元
- Cambridge University Press (CUP)
Abstract
Single-cell analysis is important to understand how individual cells work and respond at the cell population level. Experimental single-cell isolation techniques, including dilution, fluorescence-activated cell sorting, microfluidics, and micromanipulation, have been developed in recent decades. However, such applications typically require large cell populations and skilled professionals. Additionally, these methods are unsuitable for sequential analysis before and after cell isolation. In this study, we propose a method for target cell isolation using automated infrared laser-mediated disruption of pollen grains in pollen populations. Germination of the target pollen was observed at the same location as that before laser irradiation, and germinated pollen grains were enriched in the cell population. Pollination of laser-irradiated bulk pollen populations also showed that the target pollen preferentially germinated on the stigma. This method is expected to facilitate physiological analyses of target cells at the single-cell level and effectively produce seeds derived from target pollen.
Single-cell analysis is important to understand how individual cells work and respond at the cell population level. Experimental single-cell isolation techniques, including dilution, fluorescence-activated cell sorting, microfluidics, and micromanipulation, have been developed in recent decades. However, such applications typically require large cell populations and skilled professionals. Additionally, these methods are unsuitable for sequential analysis before and after cell isolation. In this study, we propose a method for target cell isolation using automated infrared laser-mediated disruption of pollen grains in pollen populations. Germination of the target pollen was observed at the same location as that before laser irradiation, and germinated pollen grains were enriched in the cell population. Pollination of laser-irradiated bulk pollen populations also showed that the target pollen preferentially germinated on the stigma. This method is expected to facilitate physiological analyses of target cells at the single-cell level and effectively produce seeds derived from target pollen.
- リンク情報
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- DOI
- https://doi.org/10.1017/qpb.2022.24
- 共同研究・競争的資金等の研究課題
- 根と葉をつなぐ 植物空腹・指令シグナルの可視化と制御(研究代表者:田畑亮)
- 共同研究・競争的資金等の研究課題
- 細胞運命操作による植物生殖システムのリモデリング
- 共同研究・競争的資金等の研究課題
- 1細胞追跡による花粉の精細胞の運命と受精能を決定するメカニズムの解明
- 共同研究・競争的資金等の研究課題
- 花粉による新植物育種技術の開発
- URL
- https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S2632882822000248
- URL
- https://www.microscope.healthcare.nikon.com/ja_JP/resources/application-notes/automatic-isolation-of-pollen-using-nis-elements-general-analysis-ga-and-jobs-imaging-workflow-tools
- ID情報
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- DOI : 10.1017/qpb.2022.24
- eISSN : 2632-8828